Results 21 - 26 of 26 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
26809 | 3' | -54.4 | NC_005808.1 | + | 9858 | 0.67 | 0.653267 |
Target: 5'- cGUCGaUGAGCGU---GGCaCCGGCGCGc -3' miRNA: 3'- -UAGUaGCUUGCAuagCCG-GGCUGCGC- -5' |
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26809 | 3' | -54.4 | NC_005808.1 | + | 27195 | 0.67 | 0.653267 |
Target: 5'- -gCAUCGGuCGUccggcaucuucGcCGGCCCGcACGCGg -3' miRNA: 3'- uaGUAGCUuGCA-----------UaGCCGGGC-UGCGC- -5' |
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26809 | 3' | -54.4 | NC_005808.1 | + | 26400 | 0.66 | 0.697789 |
Target: 5'- cGUCuUCGGgcgaauggcGCGUggcGUCGGUgaacuCCGACGCGg -3' miRNA: 3'- -UAGuAGCU---------UGCA---UAGCCG-----GGCUGCGC- -5' |
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26809 | 3' | -54.4 | NC_005808.1 | + | 18823 | 0.66 | 0.707687 |
Target: 5'- cAUCuUCGuagccgcGGCGcAUCGGCCCgGugGCGc -3' miRNA: 3'- -UAGuAGC-------UUGCaUAGCCGGG-CugCGC- -5' |
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26809 | 3' | -54.4 | NC_005808.1 | + | 40351 | 0.66 | 0.71316 |
Target: 5'- -gCGUCGAACugccggccaaGGCCCGGCGUu -3' miRNA: 3'- uaGUAGCUUGcauag-----CCGGGCUGCGc -5' |
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26809 | 3' | -54.4 | NC_005808.1 | + | 25208 | 0.66 | 0.719699 |
Target: 5'- --aAUCGaAGCGgugcGUCGGCgCGAUGCa -3' miRNA: 3'- uagUAGC-UUGCa---UAGCCGgGCUGCGc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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