Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 26809 | 3' | -54.4 | NC_005808.1 | + | 15428 | 0.69 | 0.540858 |
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Target: 5'- gGUCGUCGAggcauccGCGUG-CGGCgCGugGgGg -3' miRNA: 3'- -UAGUAGCU-------UGCAUaGCCGgGCugCgC- -5' |
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| 26809 | 3' | -54.4 | NC_005808.1 | + | 11410 | 0.69 | 0.509587 |
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Target: 5'- cGUCAggcaGAACGUGcgCGGCCCG-UGCu -3' miRNA: 3'- -UAGUag--CUUGCAUa-GCCGGGCuGCGc -5' |
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| 26809 | 3' | -54.4 | NC_005808.1 | + | 24115 | 0.7 | 0.488468 |
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Target: 5'- gGUCAUCacgguGGACGaAUCGG-CCGACGCc -3' miRNA: 3'- -UAGUAG-----CUUGCaUAGCCgGGCUGCGc -5' |
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| 26809 | 3' | -54.4 | NC_005808.1 | + | 9981 | 0.7 | 0.45759 |
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Target: 5'- -aCcgCG-GCGUcgCGGCCCuuGACGCGg -3' miRNA: 3'- uaGuaGCuUGCAuaGCCGGG--CUGCGC- -5' |
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| 26809 | 3' | -54.4 | NC_005808.1 | + | 6089 | 0.72 | 0.354277 |
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Target: 5'- aGUCGUCGAGCG---CGGCCUuGCGCa -3' miRNA: 3'- -UAGUAGCUUGCauaGCCGGGcUGCGc -5' |
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| 26809 | 3' | -54.4 | NC_005808.1 | + | 25208 | 0.66 | 0.719699 |
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Target: 5'- --aAUCGaAGCGgugcGUCGGCgCGAUGCa -3' miRNA: 3'- uagUAGC-UUGCa---UAGCCGgGCUGCGc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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