Results 1 - 20 of 40 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
26811 | 3' | -53.6 | NC_005808.1 | + | 25377 | 0.66 | 0.727137 |
Target: 5'- gGGcAGGCGCCAGAAGCCCUuuUGCgaGu- -3' miRNA: 3'- -UC-UUUGUGGUUUUCGGGG--GCGgaCug -5' |
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26811 | 3' | -53.6 | NC_005808.1 | + | 24551 | 0.66 | 0.693549 |
Target: 5'- ---cGCGCCGguAAGGCCCCCaagcagcccGCC-GACa -3' miRNA: 3'- ucuuUGUGGU--UUUCGGGGG---------CGGaCUG- -5' |
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26811 | 3' | -53.6 | NC_005808.1 | + | 19710 | 0.66 | 0.727137 |
Target: 5'- uGGAAGCugCAuaGAGGCgccgcgcaucgCCCUGCUUGGg -3' miRNA: 3'- -UCUUUGugGU--UUUCG-----------GGGGCGGACUg -5' |
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26811 | 3' | -53.6 | NC_005808.1 | + | 35214 | 0.66 | 0.727137 |
Target: 5'- uGGAAGguCGCCGc-GGUgCCCGCCgcugGACg -3' miRNA: 3'- -UCUUU--GUGGUuuUCGgGGGCGGa---CUG- -5' |
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26811 | 3' | -53.6 | NC_005808.1 | + | 31181 | 0.66 | 0.716026 |
Target: 5'- uGAAGCAC---GGGCCgcgcacguUCUGCCUGACg -3' miRNA: 3'- uCUUUGUGguuUUCGG--------GGGCGGACUG- -5' |
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26811 | 3' | -53.6 | NC_005808.1 | + | 35088 | 0.66 | 0.727137 |
Target: 5'- -cGGACGCCGAAAGCgCgCGCCg--- -3' miRNA: 3'- ucUUUGUGGUUUUCGgGgGCGGacug -5' |
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26811 | 3' | -53.6 | NC_005808.1 | + | 24149 | 0.66 | 0.704827 |
Target: 5'- ---cGCGCCu--GGCCCagCGCCUcGACg -3' miRNA: 3'- ucuuUGUGGuuuUCGGGg-GCGGA-CUG- -5' |
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26811 | 3' | -53.6 | NC_005808.1 | + | 29270 | 0.66 | 0.670814 |
Target: 5'- cGGcGACcuGCCGAAAGCCUacgCCGCCU-ACa -3' miRNA: 3'- -UCuUUG--UGGUUUUCGGG---GGCGGAcUG- -5' |
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26811 | 3' | -53.6 | NC_005808.1 | + | 15158 | 0.66 | 0.704827 |
Target: 5'- --cAGCGCC-AGGGCCUgcggCCGCCgGGCg -3' miRNA: 3'- ucuUUGUGGuUUUCGGG----GGCGGaCUG- -5' |
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26811 | 3' | -53.6 | NC_005808.1 | + | 13535 | 0.66 | 0.727137 |
Target: 5'- ---cGCGCCGAAcuGUCggCCGCCUGACu -3' miRNA: 3'- ucuuUGUGGUUUu-CGGg-GGCGGACUG- -5' |
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26811 | 3' | -53.6 | NC_005808.1 | + | 31378 | 0.66 | 0.682207 |
Target: 5'- cGGAAGCugCucAAGGCguugaaCCCCGaCUGGCa -3' miRNA: 3'- -UCUUUGugGu-UUUCG------GGGGCgGACUG- -5' |
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26811 | 3' | -53.6 | NC_005808.1 | + | 3788 | 0.66 | 0.682207 |
Target: 5'- -----uGCCGAAAGCCagCGCCUGGa -3' miRNA: 3'- ucuuugUGGUUUUCGGggGCGGACUg -5' |
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26811 | 3' | -53.6 | NC_005808.1 | + | 17989 | 0.67 | 0.613488 |
Target: 5'- cGuuACACCA--AGUCgCCGCCgGACu -3' miRNA: 3'- uCuuUGUGGUuuUCGGgGGCGGaCUG- -5' |
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26811 | 3' | -53.6 | NC_005808.1 | + | 30122 | 0.67 | 0.647919 |
Target: 5'- uGAGgccGCGCCAGAAcacGCCgCCgGCCUGcACc -3' miRNA: 3'- uCUU---UGUGGUUUU---CGG-GGgCGGAC-UG- -5' |
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26811 | 3' | -53.6 | NC_005808.1 | + | 17937 | 0.67 | 0.613488 |
Target: 5'- cGGggGCAC--GAAGCCCCCGUa---- -3' miRNA: 3'- -UCuuUGUGguUUUCGGGGGCGgacug -5' |
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26811 | 3' | -53.6 | NC_005808.1 | + | 11576 | 0.67 | 0.613488 |
Target: 5'- cGAAGCcauGCCGcccAGGGCCgCCGgCUGGCc -3' miRNA: 3'- uCUUUG---UGGU---UUUCGGgGGCgGACUG- -5' |
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26811 | 3' | -53.6 | NC_005808.1 | + | 35788 | 0.67 | 0.647919 |
Target: 5'- cGAAacccGCGCCGu--GUCCCUGgCUGACc -3' miRNA: 3'- uCUU----UGUGGUuuuCGGGGGCgGACUG- -5' |
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26811 | 3' | -53.6 | NC_005808.1 | + | 19644 | 0.67 | 0.636443 |
Target: 5'- cAGGAAUACCAGAAGCagaCCgaaGCCUa-- -3' miRNA: 3'- -UCUUUGUGGUUUUCGg--GGg--CGGAcug -5' |
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26811 | 3' | -53.6 | NC_005808.1 | + | 16682 | 0.67 | 0.636443 |
Target: 5'- uGGGcCACCGcAAGCUgcaagucgaaaCCCGCCUGAa -3' miRNA: 3'- uCUUuGUGGUuUUCGG-----------GGGCGGACUg -5' |
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26811 | 3' | -53.6 | NC_005808.1 | + | 18883 | 0.67 | 0.636443 |
Target: 5'- cGAGGCACU---GGCCCgCGaCCUGGg -3' miRNA: 3'- uCUUUGUGGuuuUCGGGgGC-GGACUg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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