Results 1 - 20 of 23 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
27018 | 5' | -57.6 | NC_005832.1 | + | 35700 | 0.66 | 0.766706 |
Target: 5'- cGCcGUGGGCGccuCUGCgUCUAGguGGg -3' miRNA: 3'- -UGcCACCUGUu--GACGgAGGUCguCCg -5' |
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27018 | 5' | -57.6 | NC_005832.1 | + | 75349 | 0.66 | 0.766706 |
Target: 5'- gGCGGcguaGGAUccccuccuGACcuUGCCcugCCAGUAGGCa -3' miRNA: 3'- -UGCCa---CCUG--------UUG--ACGGa--GGUCGUCCG- -5' |
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27018 | 5' | -57.6 | NC_005832.1 | + | 5915 | 0.66 | 0.766706 |
Target: 5'- gACGGggacgaGGGCAACcucuaCCUCCcGCAGGg -3' miRNA: 3'- -UGCCa-----CCUGUUGac---GGAGGuCGUCCg -5' |
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27018 | 5' | -57.6 | NC_005832.1 | + | 54384 | 0.66 | 0.757009 |
Target: 5'- cACGGUGGACAGg-GUCggCCc-CAGGCc -3' miRNA: 3'- -UGCCACCUGUUgaCGGa-GGucGUCCG- -5' |
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27018 | 5' | -57.6 | NC_005832.1 | + | 10814 | 0.66 | 0.756033 |
Target: 5'- -aGGUGGGuCAugccGgUGCCUCcCAGCAccuccccGGCa -3' miRNA: 3'- ugCCACCU-GU----UgACGGAG-GUCGU-------CCG- -5' |
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27018 | 5' | -57.6 | NC_005832.1 | + | 62102 | 0.66 | 0.747198 |
Target: 5'- gACGGggaUGGAC-ACgggGCCggucCCAGuUAGGCa -3' miRNA: 3'- -UGCC---ACCUGuUGa--CGGa---GGUC-GUCCG- -5' |
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27018 | 5' | -57.6 | NC_005832.1 | + | 39630 | 0.67 | 0.686468 |
Target: 5'- uCGGUGGACGugauaCUUCCAGuCGGGUu -3' miRNA: 3'- uGCCACCUGUugac-GGAGGUC-GUCCG- -5' |
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27018 | 5' | -57.6 | NC_005832.1 | + | 53341 | 0.67 | 0.676126 |
Target: 5'- aACGuGUGGAaGGCUGCC-CUGGCcuacaGGGCc -3' miRNA: 3'- -UGC-CACCUgUUGACGGaGGUCG-----UCCG- -5' |
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27018 | 5' | -57.6 | NC_005832.1 | + | 71158 | 0.68 | 0.65534 |
Target: 5'- -gGGcUGGAgAg--GCuCUCCGGCGGGCa -3' miRNA: 3'- ugCC-ACCUgUugaCG-GAGGUCGUCCG- -5' |
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27018 | 5' | -57.6 | NC_005832.1 | + | 11888 | 0.68 | 0.644914 |
Target: 5'- gGCGGUG-AgGGCUccGUCUCCcauAGCGGGCc -3' miRNA: 3'- -UGCCACcUgUUGA--CGGAGG---UCGUCCG- -5' |
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27018 | 5' | -57.6 | NC_005832.1 | + | 26600 | 0.68 | 0.603187 |
Target: 5'- -aGGUGG-CGAggGCCgCCGGguGGCu -3' miRNA: 3'- ugCCACCuGUUgaCGGaGGUCguCCG- -5' |
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27018 | 5' | -57.6 | NC_005832.1 | + | 39796 | 0.69 | 0.592791 |
Target: 5'- aGCGGUGGGucCAACcgGUC-CUAGCGGGg -3' miRNA: 3'- -UGCCACCU--GUUGa-CGGaGGUCGUCCg -5' |
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27018 | 5' | -57.6 | NC_005832.1 | + | 43270 | 0.69 | 0.551578 |
Target: 5'- gACGGUGGcCAGCacgGCCUCCcu-GGGUg -3' miRNA: 3'- -UGCCACCuGUUGa--CGGAGGucgUCCG- -5' |
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27018 | 5' | -57.6 | NC_005832.1 | + | 66821 | 0.69 | 0.541402 |
Target: 5'- cACGGUcccGGccGCGGCcuggGCUUCCAGCAGaGCc -3' miRNA: 3'- -UGCCA---CC--UGUUGa---CGGAGGUCGUC-CG- -5' |
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27018 | 5' | -57.6 | NC_005832.1 | + | 7589 | 0.7 | 0.531289 |
Target: 5'- cACGGUGGGaaaGACUgcgaGCCUCgAGCuGGa -3' miRNA: 3'- -UGCCACCUg--UUGA----CGGAGgUCGuCCg -5' |
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27018 | 5' | -57.6 | NC_005832.1 | + | 66374 | 0.7 | 0.531289 |
Target: 5'- gACGG-GGGCAugUcauaGCCUCCAG--GGCa -3' miRNA: 3'- -UGCCaCCUGUugA----CGGAGGUCguCCG- -5' |
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27018 | 5' | -57.6 | NC_005832.1 | + | 46637 | 0.7 | 0.501388 |
Target: 5'- gAUGGUGGGCAugACUcCCUUUuucguGGCGGGCu -3' miRNA: 3'- -UGCCACCUGU--UGAcGGAGG-----UCGUCCG- -5' |
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27018 | 5' | -57.6 | NC_005832.1 | + | 50935 | 0.71 | 0.453307 |
Target: 5'- gACGGUaGGACugGAUcgGCCggUCCAGuCAGGCg -3' miRNA: 3'- -UGCCA-CCUG--UUGa-CGG--AGGUC-GUCCG- -5' |
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27018 | 5' | -57.6 | NC_005832.1 | + | 34278 | 0.73 | 0.370279 |
Target: 5'- uGCGGUGGACAugUuCCUCUugcugauccacucGUAGGCa -3' miRNA: 3'- -UGCCACCUGUugAcGGAGGu------------CGUCCG- -5' |
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27018 | 5' | -57.6 | NC_005832.1 | + | 10596 | 0.73 | 0.333611 |
Target: 5'- -gGGUGGAgGAgaGCCaCCGGCAGGg -3' miRNA: 3'- ugCCACCUgUUgaCGGaGGUCGUCCg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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