Results 1 - 20 of 36 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
27021 | 5' | -51.4 | NC_005832.1 | + | 103831 | 0.66 | 0.978298 |
Target: 5'- -gGAGGCCuGGUUAgucCCCGGGGu--- -3' miRNA: 3'- cgCUCCGG-CUAGUau-GGGUCCUuuuc -5' |
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27021 | 5' | -51.4 | NC_005832.1 | + | 79292 | 0.66 | 0.978298 |
Target: 5'- aGgGAGGCCGcUCc-ACCCAGGc---- -3' miRNA: 3'- -CgCUCCGGCuAGuaUGGGUCCuuuuc -5' |
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27021 | 5' | -51.4 | NC_005832.1 | + | 39470 | 0.66 | 0.975757 |
Target: 5'- gGCccuGGUCGccGUCGUcCCCGGGggGGGu -3' miRNA: 3'- -CGcu-CCGGC--UAGUAuGGGUCCuuUUC- -5' |
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27021 | 5' | -51.4 | NC_005832.1 | + | 39359 | 0.66 | 0.975757 |
Target: 5'- -aGGGGCCcaggg-GCCCAGGGGAGc -3' miRNA: 3'- cgCUCCGGcuaguaUGGGUCCUUUUc -5' |
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27021 | 5' | -51.4 | NC_005832.1 | + | 44330 | 0.66 | 0.973005 |
Target: 5'- gGUGAGGCUGAggaccCCCAGGcacccAGAGa -3' miRNA: 3'- -CGCUCCGGCUaguauGGGUCCu----UUUC- -5' |
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27021 | 5' | -51.4 | NC_005832.1 | + | 52802 | 0.66 | 0.966837 |
Target: 5'- cCGGGGCCaugC-UGCCCAGGGGc-- -3' miRNA: 3'- cGCUCCGGcuaGuAUGGGUCCUUuuc -5' |
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27021 | 5' | -51.4 | NC_005832.1 | + | 19730 | 0.66 | 0.966837 |
Target: 5'- -gGGGGaCCG-UCAgGCCCAGGGGc-- -3' miRNA: 3'- cgCUCC-GGCuAGUaUGGGUCCUUuuc -5' |
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27021 | 5' | -51.4 | NC_005832.1 | + | 102366 | 0.67 | 0.963405 |
Target: 5'- cCGAGG-CGGUCGUAgCCAuGGAGGc- -3' miRNA: 3'- cGCUCCgGCUAGUAUgGGU-CCUUUuc -5' |
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27021 | 5' | -51.4 | NC_005832.1 | + | 98834 | 0.67 | 0.955816 |
Target: 5'- gGCGgaAGGCaccaGAUCAUGCCCGGc----- -3' miRNA: 3'- -CGC--UCCGg---CUAGUAUGGGUCcuuuuc -5' |
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27021 | 5' | -51.4 | NC_005832.1 | + | 97634 | 0.67 | 0.955816 |
Target: 5'- aGCuGGGuuGc-CAUGCCCAGGAAccGg -3' miRNA: 3'- -CGcUCCggCuaGUAUGGGUCCUUuuC- -5' |
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27021 | 5' | -51.4 | NC_005832.1 | + | 94781 | 0.67 | 0.951647 |
Target: 5'- cGCGAgGGCCGA-CGcgGCCUgaucGGGAGAAc -3' miRNA: 3'- -CGCU-CCGGCUaGUa-UGGG----UCCUUUUc -5' |
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27021 | 5' | -51.4 | NC_005832.1 | + | 41807 | 0.67 | 0.945844 |
Target: 5'- cGUGAGGUaCGAgccccCGUGCCCagacggcaaacccuGGGAGAAGc -3' miRNA: 3'- -CGCUCCG-GCUa----GUAUGGG--------------UCCUUUUC- -5' |
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27021 | 5' | -51.4 | NC_005832.1 | + | 42508 | 0.68 | 0.942538 |
Target: 5'- -aGAcGCCGAUCGgGCCUcuguGGAAGAGg -3' miRNA: 3'- cgCUcCGGCUAGUaUGGGu---CCUUUUC- -5' |
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27021 | 5' | -51.4 | NC_005832.1 | + | 49588 | 0.68 | 0.942538 |
Target: 5'- -gGAGGUC---CAUGCCCGGGAAGc- -3' miRNA: 3'- cgCUCCGGcuaGUAUGGGUCCUUUuc -5' |
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27021 | 5' | -51.4 | NC_005832.1 | + | 11555 | 0.68 | 0.942538 |
Target: 5'- aGCGuGGCCGAaaggGCCaAGGAAAAa -3' miRNA: 3'- -CGCuCCGGCUaguaUGGgUCCUUUUc -5' |
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27021 | 5' | -51.4 | NC_005832.1 | + | 11308 | 0.68 | 0.926907 |
Target: 5'- cGCgGAGGCC-AUCAUGgCCAGGu---- -3' miRNA: 3'- -CG-CUCCGGcUAGUAUgGGUCCuuuuc -5' |
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27021 | 5' | -51.4 | NC_005832.1 | + | 23883 | 0.68 | 0.926907 |
Target: 5'- uGCGAGGuuGGUCGUGaccuCCCAGu----- -3' miRNA: 3'- -CGCUCCggCUAGUAU----GGGUCcuuuuc -5' |
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27021 | 5' | -51.4 | NC_005832.1 | + | 29635 | 0.68 | 0.921165 |
Target: 5'- aGgGAGGCU----AUAUCCAGGGGAGGg -3' miRNA: 3'- -CgCUCCGGcuagUAUGGGUCCUUUUC- -5' |
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27021 | 5' | -51.4 | NC_005832.1 | + | 18443 | 0.68 | 0.920576 |
Target: 5'- aGCGGGGCCaGAUUuucUGCCCAcauucuuGGGAGGc -3' miRNA: 3'- -CGCUCCGG-CUAGu--AUGGGU-------CCUUUUc -5' |
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27021 | 5' | -51.4 | NC_005832.1 | + | 84141 | 0.69 | 0.908888 |
Target: 5'- aCGGGaGCgGAcagCAUcgcGCCCAGGGAAGGu -3' miRNA: 3'- cGCUC-CGgCUa--GUA---UGGGUCCUUUUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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