Results 1 - 20 of 26 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
27025 | 5' | -54.4 | NC_005832.1 | + | 2653 | 0.66 | 0.903983 |
Target: 5'- uUgUCGCgGAGGAGGGcCUGUG-GACu -3' miRNA: 3'- uGgGGUGgCUUUUCCCaGACACuCUG- -5' |
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27025 | 5' | -54.4 | NC_005832.1 | + | 654 | 0.66 | 0.903983 |
Target: 5'- gACCCCGCCG---GGGG-CUGgUGcAGAa -3' miRNA: 3'- -UGGGGUGGCuuuUCCCaGAC-AC-UCUg -5' |
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27025 | 5' | -54.4 | NC_005832.1 | + | 47425 | 0.66 | 0.897353 |
Target: 5'- gACCUUAUgGAGAAGcuccaGGUCUggGUGGGACu -3' miRNA: 3'- -UGGGGUGgCUUUUC-----CCAGA--CACUCUG- -5' |
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27025 | 5' | -54.4 | NC_005832.1 | + | 95720 | 0.66 | 0.890473 |
Target: 5'- gUCCUGCCGGuccuAGGGUUccugGUGGGAUc -3' miRNA: 3'- uGGGGUGGCUuu--UCCCAGa---CACUCUG- -5' |
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27025 | 5' | -54.4 | NC_005832.1 | + | 95510 | 0.66 | 0.890473 |
Target: 5'- gUCCUGCCGGuccuAGGGUUccugGUGGGAUc -3' miRNA: 3'- uGGGGUGGCUuu--UCCCAGa---CACUCUG- -5' |
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27025 | 5' | -54.4 | NC_005832.1 | + | 54025 | 0.66 | 0.883349 |
Target: 5'- gACCCCuuuCUGguGA-GGUCUGggaGAGACg -3' miRNA: 3'- -UGGGGu--GGCuuUUcCCAGACa--CUCUG- -5' |
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27025 | 5' | -54.4 | NC_005832.1 | + | 54260 | 0.66 | 0.883349 |
Target: 5'- cCCCCGugUCGuguAGGGUCcucuugucUGUGAGGCc -3' miRNA: 3'- uGGGGU--GGCuuuUCCCAG--------ACACUCUG- -5' |
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27025 | 5' | -54.4 | NC_005832.1 | + | 4755 | 0.67 | 0.871452 |
Target: 5'- cCCCCGCCGAucccauccuGGGGUCguaaacgGaccccgucucugccuUGAGGCa -3' miRNA: 3'- uGGGGUGGCUuu-------UCCCAGa------C---------------ACUCUG- -5' |
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27025 | 5' | -54.4 | NC_005832.1 | + | 49375 | 0.67 | 0.860555 |
Target: 5'- uGCa-CACCGAcauGGGG-CUGUGAGAa -3' miRNA: 3'- -UGggGUGGCUuu-UCCCaGACACUCUg -5' |
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27025 | 5' | -54.4 | NC_005832.1 | + | 35270 | 0.67 | 0.85736 |
Target: 5'- gGCCCCuguCCGAAGgcAGGGacaggCUGguucucuuugcggGAGACg -3' miRNA: 3'- -UGGGGu--GGCUUU--UCCCa----GACa------------CUCUG- -5' |
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27025 | 5' | -54.4 | NC_005832.1 | + | 32724 | 0.67 | 0.852503 |
Target: 5'- cGCCUCGuCCGGggagaggcuGGAGGuGUCUGcggGAGGCa -3' miRNA: 3'- -UGGGGU-GGCU---------UUUCC-CAGACa--CUCUG- -5' |
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27025 | 5' | -54.4 | NC_005832.1 | + | 32342 | 0.68 | 0.809168 |
Target: 5'- uCCCCGCCGGggcugGAGGGGgauccUUGUG-GGCu -3' miRNA: 3'- uGGGGUGGCU-----UUUCCCa----GACACuCUG- -5' |
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27025 | 5' | -54.4 | NC_005832.1 | + | 52179 | 0.68 | 0.790558 |
Target: 5'- gGCCCUcUUGAcguAGGGUCUG-GGGACc -3' miRNA: 3'- -UGGGGuGGCUuu-UCCCAGACaCUCUG- -5' |
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27025 | 5' | -54.4 | NC_005832.1 | + | 100357 | 0.69 | 0.7684 |
Target: 5'- cCCCCGCUG-GAGGGGauguaaaugcuuacUCUGUGuGGCc -3' miRNA: 3'- uGGGGUGGCuUUUCCC--------------AGACACuCUG- -5' |
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27025 | 5' | -54.4 | NC_005832.1 | + | 59258 | 0.69 | 0.761513 |
Target: 5'- aACCCU-CCGAGguuGAGGGUuggaaaCUGUGuGACa -3' miRNA: 3'- -UGGGGuGGCUU---UUCCCA------GACACuCUG- -5' |
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27025 | 5' | -54.4 | NC_005832.1 | + | 80033 | 0.69 | 0.761513 |
Target: 5'- cCCCCAUCGccGAAGGGUCc--GAGGCc -3' miRNA: 3'- uGGGGUGGCu-UUUCCCAGacaCUCUG- -5' |
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27025 | 5' | -54.4 | NC_005832.1 | + | 64962 | 0.69 | 0.75157 |
Target: 5'- cGCCUCcauACCGG--AGGGUgUGUGGGAa -3' miRNA: 3'- -UGGGG---UGGCUuuUCCCAgACACUCUg -5' |
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27025 | 5' | -54.4 | NC_005832.1 | + | 28336 | 0.69 | 0.741514 |
Target: 5'- uCCCCuCCGAggggggAAAGGGUUUacGUGAGAa -3' miRNA: 3'- uGGGGuGGCU------UUUCCCAGA--CACUCUg -5' |
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27025 | 5' | -54.4 | NC_005832.1 | + | 83391 | 0.69 | 0.731354 |
Target: 5'- gGCCCCGCCaccucAAGGGUaUGgaggGAGACc -3' miRNA: 3'- -UGGGGUGGcuu--UUCCCAgACa---CUCUG- -5' |
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27025 | 5' | -54.4 | NC_005832.1 | + | 36522 | 0.69 | 0.731354 |
Target: 5'- cGCCCUguacaACUGGAuGGGGUCgg-GAGACu -3' miRNA: 3'- -UGGGG-----UGGCUUuUCCCAGacaCUCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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