Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 27025 | 5' | -54.4 | NC_005832.1 | + | 35176 | 0.7 | 0.721102 |
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Target: 5'- gGCCUCugUGGGAgacagccucAGGGUCUGgaccguUGAGGCu -3' miRNA: 3'- -UGGGGugGCUUU---------UCCCAGAC------ACUCUG- -5' |
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| 27025 | 5' | -54.4 | NC_005832.1 | + | 105109 | 0.71 | 0.636966 |
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Target: 5'- aACCUCACCGuugagggcguAGAGGGUUUcGUGAGGu -3' miRNA: 3'- -UGGGGUGGCu---------UUUCCCAGA-CACUCUg -5' |
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| 27025 | 5' | -54.4 | NC_005832.1 | + | 53900 | 0.77 | 0.315581 |
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Target: 5'- gACCUCACCaGAAAGGGGUcCUGaagGAGGCc -3' miRNA: 3'- -UGGGGUGG-CUUUUCCCA-GACa--CUCUG- -5' |
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| 27025 | 5' | -54.4 | NC_005832.1 | + | 104898 | 0.78 | 0.279747 |
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Target: 5'- cACCCCuccguCgGAAAAGGGUCUuaucGUGGGGCa -3' miRNA: 3'- -UGGGGu----GgCUUUUCCCAGA----CACUCUG- -5' |
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| 27025 | 5' | -54.4 | NC_005832.1 | + | 5210 | 0.82 | 0.155302 |
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Target: 5'- gACCCCGCCGucGAGGGUCUG-GuGGCc -3' miRNA: 3'- -UGGGGUGGCuuUUCCCAGACaCuCUG- -5' |
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| 27025 | 5' | -54.4 | NC_005832.1 | + | 6516 | 1.1 | 0.002271 |
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Target: 5'- uACCCCACCGAAAAGGGUCUGUGAGACa -3' miRNA: 3'- -UGGGGUGGCUUUUCCCAGACACUCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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