Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 27025 | 5' | -54.4 | NC_005832.1 | + | 80033 | 0.69 | 0.761513 |
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Target: 5'- cCCCCAUCGccGAAGGGUCc--GAGGCc -3' miRNA: 3'- uGGGGUGGCu-UUUCCCAGacaCUCUG- -5' |
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| 27025 | 5' | -54.4 | NC_005832.1 | + | 36522 | 0.69 | 0.731354 |
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Target: 5'- cGCCCUguacaACUGGAuGGGGUCgg-GAGACu -3' miRNA: 3'- -UGGGG-----UGGCUUuUCCCAGacaCUCUG- -5' |
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| 27025 | 5' | -54.4 | NC_005832.1 | + | 83391 | 0.69 | 0.731354 |
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Target: 5'- gGCCCCGCCaccucAAGGGUaUGgaggGAGACc -3' miRNA: 3'- -UGGGGUGGcuu--UUCCCAgACa---CUCUG- -5' |
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| 27025 | 5' | -54.4 | NC_005832.1 | + | 35176 | 0.7 | 0.721102 |
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Target: 5'- gGCCUCugUGGGAgacagccucAGGGUCUGgaccguUGAGGCu -3' miRNA: 3'- -UGGGGugGCUUU---------UCCCAGAC------ACUCUG- -5' |
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| 27025 | 5' | -54.4 | NC_005832.1 | + | 6516 | 1.1 | 0.002271 |
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Target: 5'- uACCCCACCGAAAAGGGUCUGUGAGACa -3' miRNA: 3'- -UGGGGUGGCUUUUCCCAGACACUCUG- -5' |
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| 27025 | 5' | -54.4 | NC_005832.1 | + | 654 | 0.66 | 0.903983 |
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Target: 5'- gACCCCGCCG---GGGG-CUGgUGcAGAa -3' miRNA: 3'- -UGGGGUGGCuuuUCCCaGAC-AC-UCUg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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