Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 27025 | 5' | -54.4 | NC_005832.1 | + | 83391 | 0.69 | 0.731354 |
|
Target: 5'- gGCCCCGCCaccucAAGGGUaUGgaggGAGACc -3' miRNA: 3'- -UGGGGUGGcuu--UUCCCAgACa---CUCUG- -5' |
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| 27025 | 5' | -54.4 | NC_005832.1 | + | 95510 | 0.66 | 0.890473 |
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Target: 5'- gUCCUGCCGGuccuAGGGUUccugGUGGGAUc -3' miRNA: 3'- uGGGGUGGCUuu--UCCCAGa---CACUCUG- -5' |
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| 27025 | 5' | -54.4 | NC_005832.1 | + | 95720 | 0.66 | 0.890473 |
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Target: 5'- gUCCUGCCGGuccuAGGGUUccugGUGGGAUc -3' miRNA: 3'- uGGGGUGGCUuu--UCCCAGa---CACUCUG- -5' |
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| 27025 | 5' | -54.4 | NC_005832.1 | + | 100357 | 0.69 | 0.7684 |
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Target: 5'- cCCCCGCUG-GAGGGGauguaaaugcuuacUCUGUGuGGCc -3' miRNA: 3'- uGGGGUGGCuUUUCCC--------------AGACACuCUG- -5' |
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| 27025 | 5' | -54.4 | NC_005832.1 | + | 104898 | 0.78 | 0.279747 |
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Target: 5'- cACCCCuccguCgGAAAAGGGUCUuaucGUGGGGCa -3' miRNA: 3'- -UGGGGu----GgCUUUUCCCAGA----CACUCUG- -5' |
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| 27025 | 5' | -54.4 | NC_005832.1 | + | 105109 | 0.71 | 0.636966 |
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Target: 5'- aACCUCACCGuugagggcguAGAGGGUUUcGUGAGGu -3' miRNA: 3'- -UGGGGUGGCu---------UUUCCCAGA-CACUCUg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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