Results 21 - 26 of 26 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
27025 | 5' | -54.4 | NC_005832.1 | + | 54260 | 0.66 | 0.883349 |
Target: 5'- cCCCCGugUCGuguAGGGUCcucuugucUGUGAGGCc -3' miRNA: 3'- uGGGGU--GGCuuuUCCCAG--------ACACUCUG- -5' |
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27025 | 5' | -54.4 | NC_005832.1 | + | 95510 | 0.66 | 0.890473 |
Target: 5'- gUCCUGCCGGuccuAGGGUUccugGUGGGAUc -3' miRNA: 3'- uGGGGUGGCUuu--UCCCAGa---CACUCUG- -5' |
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27025 | 5' | -54.4 | NC_005832.1 | + | 95720 | 0.66 | 0.890473 |
Target: 5'- gUCCUGCCGGuccuAGGGUUccugGUGGGAUc -3' miRNA: 3'- uGGGGUGGCUuu--UCCCAGa---CACUCUG- -5' |
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27025 | 5' | -54.4 | NC_005832.1 | + | 47425 | 0.66 | 0.897353 |
Target: 5'- gACCUUAUgGAGAAGcuccaGGUCUggGUGGGACu -3' miRNA: 3'- -UGGGGUGgCUUUUC-----CCAGA--CACUCUG- -5' |
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27025 | 5' | -54.4 | NC_005832.1 | + | 2653 | 0.66 | 0.903983 |
Target: 5'- uUgUCGCgGAGGAGGGcCUGUG-GACu -3' miRNA: 3'- uGgGGUGgCUUUUCCCaGACACuCUG- -5' |
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27025 | 5' | -54.4 | NC_005832.1 | + | 654 | 0.66 | 0.903983 |
Target: 5'- gACCCCGCCG---GGGG-CUGgUGcAGAa -3' miRNA: 3'- -UGGGGUGGCuuuUCCCaGAC-AC-UCUg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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