Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 27029 | 3' | -57.9 | NC_005832.1 | + | 79182 | 0.67 | 0.707326 |
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Target: 5'- gGAAgGGaGUcGAGGAGcucauggaGGCCGCCGGg -3' miRNA: 3'- gUUUgCC-CAcCUCCUC--------UCGGUGGCCg -5' |
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| 27029 | 3' | -57.9 | NC_005832.1 | + | 32167 | 0.67 | 0.707326 |
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Target: 5'- gGAGCGGc--GAGGGGAGuCCA-CGGCa -3' miRNA: 3'- gUUUGCCcacCUCCUCUC-GGUgGCCG- -5' |
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| 27029 | 3' | -57.9 | NC_005832.1 | + | 19652 | 0.67 | 0.707326 |
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Target: 5'- -cGGCGGGUGGgagacccgcagcGGGuucgaaAGCCgcaGCCGGCg -3' miRNA: 3'- guUUGCCCACC------------UCCuc----UCGG---UGGCCG- -5' |
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| 27029 | 3' | -57.9 | NC_005832.1 | + | 42522 | 0.67 | 0.707326 |
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Target: 5'- gCGGACGcGUGcugcuccauGAGGAGGGCCGCCauGCu -3' miRNA: 3'- -GUUUGCcCAC---------CUCCUCUCGGUGGc-CG- -5' |
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| 27029 | 3' | -57.9 | NC_005832.1 | + | 72358 | 0.67 | 0.717376 |
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Target: 5'- cCAGACGGaGacauUGGuaGGGAGGGCCAaCGGa -3' miRNA: 3'- -GUUUGCC-C----ACC--UCCUCUCGGUgGCCg -5' |
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| 27029 | 3' | -57.9 | NC_005832.1 | + | 2733 | 0.66 | 0.747044 |
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Target: 5'- uGAGCaGGGaGGAcaugucuauguuGGAGGGCCACCccGCg -3' miRNA: 3'- gUUUG-CCCaCCU------------CCUCUCGGUGGc-CG- -5' |
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| 27029 | 3' | -57.9 | NC_005832.1 | + | 1468 | 0.66 | 0.77673 |
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Target: 5'- ----gGGGUugccgugaggaccaaGAGGGGGGCCugUGGCu -3' miRNA: 3'- guuugCCCAc--------------CUCCUCUCGGugGCCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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