Results 21 - 27 of 27 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
27029 | 3' | -57.9 | NC_005832.1 | + | 67035 | 0.7 | 0.523703 |
Target: 5'- -cGGCGGccGUGGc-GAGGGCCGCgGGCg -3' miRNA: 3'- guUUGCC--CACCucCUCUCGGUGgCCG- -5' |
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27029 | 3' | -57.9 | NC_005832.1 | + | 26615 | 0.7 | 0.513833 |
Target: 5'- gAGACGcggccuuuaaGGUGGc-GAGGGCCGCCGGg -3' miRNA: 3'- gUUUGC----------CCACCucCUCUCGGUGGCCg -5' |
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27029 | 3' | -57.9 | NC_005832.1 | + | 75099 | 0.72 | 0.411267 |
Target: 5'- --cAUGGGaGGAGGAGuggcgGGCCACUGGg -3' miRNA: 3'- guuUGCCCaCCUCCUC-----UCGGUGGCCg -5' |
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27029 | 3' | -57.9 | NC_005832.1 | + | 91966 | 0.73 | 0.377225 |
Target: 5'- -uGGCGaGGUGGgccAGGAGcAGCCuaaaACCGGCu -3' miRNA: 3'- guUUGC-CCACC---UCCUC-UCGG----UGGCCG- -5' |
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27029 | 3' | -57.9 | NC_005832.1 | + | 10972 | 0.73 | 0.358529 |
Target: 5'- --cGCGGGUcugaccuuugccggGGAGGugcugGGAGgCACCGGCa -3' miRNA: 3'- guuUGCCCA--------------CCUCC-----UCUCgGUGGCCG- -5' |
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27029 | 3' | -57.9 | NC_005832.1 | + | 99668 | 0.74 | 0.329838 |
Target: 5'- aAGACGGGc-GAGGAGAuGCCAgCGGUu -3' miRNA: 3'- gUUUGCCCacCUCCUCU-CGGUgGCCG- -5' |
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27029 | 3' | -57.9 | NC_005832.1 | + | 10602 | 1.11 | 0.000886 |
Target: 5'- gCAAACGGGUGGAGGAGAGCCACCGGCa -3' miRNA: 3'- -GUUUGCCCACCUCCUCUCGGUGGCCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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