miRNA display CGI


Results 21 - 27 of 27 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
27029 3' -57.9 NC_005832.1 + 67035 0.7 0.523703
Target:  5'- -cGGCGGccGUGGc-GAGGGCCGCgGGCg -3'
miRNA:   3'- guUUGCC--CACCucCUCUCGGUGgCCG- -5'
27029 3' -57.9 NC_005832.1 + 26615 0.7 0.513833
Target:  5'- gAGACGcggccuuuaaGGUGGc-GAGGGCCGCCGGg -3'
miRNA:   3'- gUUUGC----------CCACCucCUCUCGGUGGCCg -5'
27029 3' -57.9 NC_005832.1 + 75099 0.72 0.411267
Target:  5'- --cAUGGGaGGAGGAGuggcgGGCCACUGGg -3'
miRNA:   3'- guuUGCCCaCCUCCUC-----UCGGUGGCCg -5'
27029 3' -57.9 NC_005832.1 + 91966 0.73 0.377225
Target:  5'- -uGGCGaGGUGGgccAGGAGcAGCCuaaaACCGGCu -3'
miRNA:   3'- guUUGC-CCACC---UCCUC-UCGG----UGGCCG- -5'
27029 3' -57.9 NC_005832.1 + 10972 0.73 0.358529
Target:  5'- --cGCGGGUcugaccuuugccggGGAGGugcugGGAGgCACCGGCa -3'
miRNA:   3'- guuUGCCCA--------------CCUCC-----UCUCgGUGGCCG- -5'
27029 3' -57.9 NC_005832.1 + 99668 0.74 0.329838
Target:  5'- aAGACGGGc-GAGGAGAuGCCAgCGGUu -3'
miRNA:   3'- gUUUGCCCacCUCCUCU-CGGUgGCCG- -5'
27029 3' -57.9 NC_005832.1 + 10602 1.11 0.000886
Target:  5'- gCAAACGGGUGGAGGAGAGCCACCGGCa -3'
miRNA:   3'- -GUUUGCCCACCUCCUCUCGGUGGCCG- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.