Results 41 - 48 of 48 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 27035 | 5' | -60.3 | NC_005832.1 | + | 880 | 0.72 | 0.362275 |
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Target: 5'- gCCCCUgggugugaagGCGCugcaGCCCCCGGCGuagcAGGg -3' miRNA: 3'- -GGGGA----------UGCGucugCGGGGGCUGC----UCC- -5' |
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| 27035 | 5' | -60.3 | NC_005832.1 | + | 35142 | 0.72 | 0.346961 |
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Target: 5'- gUCCCUGCcuucggaCAGGgGCCCuCCGACGuGGa -3' miRNA: 3'- -GGGGAUGc------GUCUgCGGG-GGCUGCuCC- -5' |
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| 27035 | 5' | -60.3 | NC_005832.1 | + | 39285 | 0.72 | 0.332116 |
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Target: 5'- gCCCCgcggGCGCAGACGgaguccacguCCaCCUGGCGGGu -3' miRNA: 3'- -GGGGa---UGCGUCUGC----------GG-GGGCUGCUCc -5' |
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| 27035 | 5' | -60.3 | NC_005832.1 | + | 66047 | 0.73 | 0.317743 |
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Target: 5'- gCCCUggACGCAcuACGUCCCCG-UGAGGg -3' miRNA: 3'- gGGGA--UGCGUc-UGCGGGGGCuGCUCC- -5' |
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| 27035 | 5' | -60.3 | NC_005832.1 | + | 40343 | 0.73 | 0.303843 |
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Target: 5'- cUCCCUAuuUGCGGGCGCCauagCCCGGC-AGGa -3' miRNA: 3'- -GGGGAU--GCGUCUGCGG----GGGCUGcUCC- -5' |
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| 27035 | 5' | -60.3 | NC_005832.1 | + | 19771 | 0.74 | 0.277457 |
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Target: 5'- cCCCCgagGCGCuuucccccGGCGCCCgUgGACGAGGc -3' miRNA: 3'- -GGGGa--UGCGu-------CUGCGGG-GgCUGCUCC- -5' |
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| 27035 | 5' | -60.3 | NC_005832.1 | + | 35567 | 0.78 | 0.152164 |
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Target: 5'- cCCaCCUagACGCAGAgGCgCCCaCGGCGGGGg -3' miRNA: 3'- -GG-GGA--UGCGUCUgCG-GGG-GCUGCUCC- -5' |
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| 27035 | 5' | -60.3 | NC_005832.1 | + | 12992 | 1.1 | 0.000837 |
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Target: 5'- uCCCCUACGCAGACGCCCCCGACGAGGc -3' miRNA: 3'- -GGGGAUGCGUCUGCGGGGGCUGCUCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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