Results 21 - 30 of 30 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 27051 | 3' | -59.9 | NC_005832.1 | + | 20820 | 0.67 | 0.597563 |
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Target: 5'- --cGUACuCUGCUCCCGauccGGAAGGCa- -3' miRNA: 3'- gcaCGUGuGGCGAGGGU----CCUUCCGga -5' |
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| 27051 | 3' | -59.9 | NC_005832.1 | + | 32808 | 0.66 | 0.628311 |
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Target: 5'- -cUGCAcCACCGCcucgUCCGGGGagAGGCUg -3' miRNA: 3'- gcACGU-GUGGCGa---GGGUCCU--UCCGGa -5' |
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| 27051 | 3' | -59.9 | NC_005832.1 | + | 39918 | 0.66 | 0.638576 |
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Target: 5'- cCGguugGaCcCACCGCUCC--GGggGGCCUc -3' miRNA: 3'- -GCa---C-GuGUGGCGAGGguCCuuCCGGA- -5' |
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| 27051 | 3' | -59.9 | NC_005832.1 | + | 2643 | 0.66 | 0.638576 |
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Target: 5'- -aUGCACGuuGUUgUCgcggAGGAGGGCCUg -3' miRNA: 3'- gcACGUGUggCGAgGG----UCCUUCCGGA- -5' |
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| 27051 | 3' | -59.9 | NC_005832.1 | + | 66542 | 0.66 | 0.64781 |
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Target: 5'- ----gGCAgCGUcucccaagucccaUCCCAGGGAGGCCg -3' miRNA: 3'- gcacgUGUgGCG-------------AGGGUCCUUCCGGa -5' |
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| 27051 | 3' | -59.9 | NC_005832.1 | + | 35698 | 0.66 | 0.648836 |
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Target: 5'- cCGUGgGCGCCucuGCgUCUAGGuGGGCCa -3' miRNA: 3'- -GCACgUGUGG---CGaGGGUCCuUCCGGa -5' |
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| 27051 | 3' | -59.9 | NC_005832.1 | + | 39466 | 0.66 | 0.648836 |
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Target: 5'- -cUGguCGCCGUcgUCCCcgGGGggGGUCa -3' miRNA: 3'- gcACguGUGGCG--AGGG--UCCuuCCGGa -5' |
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| 27051 | 3' | -59.9 | NC_005832.1 | + | 77355 | 0.66 | 0.648836 |
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Target: 5'- gGUGUACGCCGCcuugUCCuCGGGc--GCCUc -3' miRNA: 3'- gCACGUGUGGCG----AGG-GUCCuucCGGA- -5' |
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| 27051 | 3' | -59.9 | NC_005832.1 | + | 38297 | 0.66 | 0.648836 |
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Target: 5'- -aUGCuCACCaGCUUUgcgugCAGGGAGGCCa -3' miRNA: 3'- gcACGuGUGG-CGAGG-----GUCCUUCCGGa -5' |
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| 27051 | 3' | -59.9 | NC_005832.1 | + | 40958 | 0.66 | 0.648836 |
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Target: 5'- aCG-GCAgGgUgGCUCCCAGGuccGGCCUg -3' miRNA: 3'- -GCaCGUgUgG-CGAGGGUCCuu-CCGGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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