miRNA display CGI


Results 1 - 17 of 17 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
27130 3' -54.1 NC_005832.1 + 22695 0.66 0.927874
Target:  5'- aCCUuuucccaaaucUGCa--CGAGCAUGUCGUAUUcGGc -3'
miRNA:   3'- -GGA-----------ACGgagGCUCGUGCAGCAUGA-CC- -5'
27130 3' -54.1 NC_005832.1 + 2247 0.67 0.897848
Target:  5'- ---gGCCagcaccaCGAGCAUGUCGUGgaGGa -3'
miRNA:   3'- ggaaCGGag-----GCUCGUGCAGCAUgaCC- -5'
27130 3' -54.1 NC_005832.1 + 62017 0.67 0.891111
Target:  5'- ---aGCCUCCGGGCugGagGgugUGGg -3'
miRNA:   3'- ggaaCGGAGGCUCGugCagCaugACC- -5'
27130 3' -54.1 NC_005832.1 + 1428 0.67 0.891111
Target:  5'- ---aGCCgUCGAGCAUGaCGUGCgGGg -3'
miRNA:   3'- ggaaCGGaGGCUCGUGCaGCAUGaCC- -5'
27130 3' -54.1 NC_005832.1 + 41946 0.67 0.884136
Target:  5'- gUUUGCCgUCUGGGCACGggggcUCGUACc-- -3'
miRNA:   3'- gGAACGG-AGGCUCGUGC-----AGCAUGacc -5'
27130 3' -54.1 NC_005832.1 + 103744 0.67 0.884136
Target:  5'- aCCUUGCCcgCC-AGCugGUgGaGCUGa -3'
miRNA:   3'- -GGAACGGa-GGcUCGugCAgCaUGACc -5'
27130 3' -54.1 NC_005832.1 + 72581 0.68 0.853964
Target:  5'- cCCUggGCUUCaGGGUcccgACGUCGUAgUGGa -3'
miRNA:   3'- -GGAa-CGGAGgCUCG----UGCAGCAUgACC- -5'
27130 3' -54.1 NC_005832.1 + 65820 0.69 0.820453
Target:  5'- -gUUGuCCUCCGuGgACGUCcccgcgcccGUGCUGGa -3'
miRNA:   3'- ggAAC-GGAGGCuCgUGCAG---------CAUGACC- -5'
27130 3' -54.1 NC_005832.1 + 98413 0.69 0.802595
Target:  5'- uCCUUGCCUCUGccgacgggaGGCAagaGUgcUGCUGGg -3'
miRNA:   3'- -GGAACGGAGGC---------UCGUg--CAgcAUGACC- -5'
27130 3' -54.1 NC_005832.1 + 24396 0.69 0.793421
Target:  5'- --aUGCCUuccCCGAGCACGUC---UUGGg -3'
miRNA:   3'- ggaACGGA---GGCUCGUGCAGcauGACC- -5'
27130 3' -54.1 NC_005832.1 + 91609 0.7 0.772716
Target:  5'- --cUGCCUUCGGGgACGUCGagacucccccuaGCUGGg -3'
miRNA:   3'- ggaACGGAGGCUCgUGCAGCa-----------UGACC- -5'
27130 3' -54.1 NC_005832.1 + 12516 0.7 0.745464
Target:  5'- uCCUgacUGCCUCCaAGCACGaCGUggACgGGg -3'
miRNA:   3'- -GGA---ACGGAGGcUCGUGCaGCA--UGaCC- -5'
27130 3' -54.1 NC_005832.1 + 65071 0.7 0.735523
Target:  5'- ---gGCgUCUGGGC-CGUCGUACUcGGa -3'
miRNA:   3'- ggaaCGgAGGCUCGuGCAGCAUGA-CC- -5'
27130 3' -54.1 NC_005832.1 + 12660 0.7 0.735523
Target:  5'- gCCgccGCC-CCGucCACGUCGUGCUuGGa -3'
miRNA:   3'- -GGaa-CGGaGGCucGUGCAGCAUGA-CC- -5'
27130 3' -54.1 NC_005832.1 + 47669 0.72 0.663863
Target:  5'- --aUGUCgaagCCGAGCACGUUGUAgccggugacgacCUGGg -3'
miRNA:   3'- ggaACGGa---GGCUCGUGCAGCAU------------GACC- -5'
27130 3' -54.1 NC_005832.1 + 97046 0.75 0.479996
Target:  5'- aCCUgGCCUCCcAGUACGaCGUGCUcGGa -3'
miRNA:   3'- -GGAaCGGAGGcUCGUGCaGCAUGA-CC- -5'
27130 3' -54.1 NC_005832.1 + 97188 1.13 0.001829
Target:  5'- gCCUUGCCUCCGAGCACGUCGUACUGGg -3'
miRNA:   3'- -GGAACGGAGGCUCGUGCAGCAUGACC- -5' 
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.