Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 27268 | 5' | -51.5 | NC_005841.1 | + | 5747 | 0.66 | 0.813301 |
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Target: 5'- aGCAGGCCAAUGCUgaauuCAUcGCUGc--- -3' miRNA: 3'- -CGUUCGGUUGCGGau---GUA-CGACcuuc -5' |
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| 27268 | 5' | -51.5 | NC_005841.1 | + | 38401 | 0.66 | 0.803258 |
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Target: 5'- gGCGAGCau-UGCaUUACGUGCUGGu-- -3' miRNA: 3'- -CGUUCGguuGCG-GAUGUACGACCuuc -5' |
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| 27268 | 5' | -51.5 | NC_005841.1 | + | 18727 | 0.67 | 0.754668 |
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Target: 5'- aGCGGGCUuuaacguGCGCuaaCUGCGgucagaagcugcaugUGCUGGAAGu -3' miRNA: 3'- -CGUUCGGu------UGCG---GAUGU---------------ACGACCUUC- -5' |
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| 27268 | 5' | -51.5 | NC_005841.1 | + | 26605 | 0.67 | 0.73923 |
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Target: 5'- aGCAAGUCcuGACGCCgcugAUGUGCUGauGGg -3' miRNA: 3'- -CGUUCGG--UUGCGGa---UGUACGACcuUC- -5' |
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| 27268 | 5' | -51.5 | NC_005841.1 | + | 33982 | 0.67 | 0.728054 |
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Target: 5'- uCAGGUCGAUG-UUGCGUGUUGGAAu -3' miRNA: 3'- cGUUCGGUUGCgGAUGUACGACCUUc -5' |
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| 27268 | 5' | -51.5 | NC_005841.1 | + | 2110 | 0.68 | 0.716771 |
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Target: 5'- gGCAGGCUGGCGCggAUaugGUGCUGGcuAAGc -3' miRNA: 3'- -CGUUCGGUUGCGgaUG---UACGACC--UUC- -5' |
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| 27268 | 5' | -51.5 | NC_005841.1 | + | 36820 | 0.74 | 0.357842 |
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Target: 5'- uGCAGucGCCAGCGCCgggGCAUggGCUGGucAGa -3' miRNA: 3'- -CGUU--CGGUUGCGGa--UGUA--CGACCu-UC- -5' |
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| 27268 | 5' | -51.5 | NC_005841.1 | + | 27542 | 1.11 | 0.001069 |
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Target: 5'- cGCAAGCCAACGCCUACAUGCUGGAAGc -3' miRNA: 3'- -CGUUCGGUUGCGGAUGUACGACCUUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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