Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 27287 | 5' | -45.1 | NC_005856.1 | + | 49404 | 0.66 | 0.999807 |
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Target: 5'- gGCAGCCAggcguaGAGC-UUcUGGUAAUGu -3' miRNA: 3'- aUGUCGGUa-----CUCGcAAaACUAUUACc -5' |
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| 27287 | 5' | -45.1 | NC_005856.1 | + | 54496 | 0.66 | 0.999749 |
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Target: 5'- -uCAGCCAggGAGCGgcaaUGAacAUGGc -3' miRNA: 3'- auGUCGGUa-CUCGCaaa-ACUauUACC- -5' |
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| 27287 | 5' | -45.1 | NC_005856.1 | + | 62579 | 0.68 | 0.998712 |
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Target: 5'- gUGCAGCC-UGuAGCGU--UGAUGcgcuUGGg -3' miRNA: 3'- -AUGUCGGuAC-UCGCAaaACUAUu---ACC- -5' |
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| 27287 | 5' | -45.1 | NC_005856.1 | + | 23237 | 0.68 | 0.998418 |
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Target: 5'- aUAUGGCCAUGAaugGCGUUg-GAUGccGGg -3' miRNA: 3'- -AUGUCGGUACU---CGCAAaaCUAUuaCC- -5' |
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| 27287 | 5' | -45.1 | NC_005856.1 | + | 82562 | 0.68 | 0.998069 |
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Target: 5'- cGCGGCCcgGA-UGUUcugGAUAAUGGc -3' miRNA: 3'- aUGUCGGuaCUcGCAAaa-CUAUUACC- -5' |
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| 27287 | 5' | -45.1 | NC_005856.1 | + | 19054 | 0.71 | 0.986098 |
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Target: 5'- uUGC-GCCAguuccugUGgcGGUGUUUUGAUGAUGGa -3' miRNA: 3'- -AUGuCGGU-------AC--UCGCAAAACUAUUACC- -5' |
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| 27287 | 5' | -45.1 | NC_005856.1 | + | 10226 | 1.1 | 0.016499 |
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Target: 5'- cUACAGCCAUGAGCGUUUUGAUAAUGGu -3' miRNA: 3'- -AUGUCGGUACUCGCAAAACUAUUACC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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