Results 21 - 27 of 27 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
27298 | 5' | -43.3 | NC_005856.1 | + | 17706 | 0.7 | 0.999026 |
Target: 5'- cGCGGugGCGAucGUGCUGUAAa--- -3' miRNA: 3'- aUGCCugUGUUuuUAUGGCGUUauag -5' |
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27298 | 5' | -43.3 | NC_005856.1 | + | 48225 | 0.7 | 0.999026 |
Target: 5'- gUGCGGACaaccGCAGAuuuccGAUAUUGCGGUAUUu -3' miRNA: 3'- -AUGCCUG----UGUUU-----UUAUGGCGUUAUAG- -5' |
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27298 | 5' | -43.3 | NC_005856.1 | + | 70444 | 0.7 | 0.998131 |
Target: 5'- aACGG-CGCAaaggcagGAAGUGCUGCAAUGg- -3' miRNA: 3'- aUGCCuGUGU-------UUUUAUGGCGUUAUag -5' |
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27298 | 5' | -43.3 | NC_005856.1 | + | 15019 | 0.71 | 0.997297 |
Target: 5'- -uCGGugGCAAAGAUAgCGCGAc--- -3' miRNA: 3'- auGCCugUGUUUUUAUgGCGUUauag -5' |
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27298 | 5' | -43.3 | NC_005856.1 | + | 81968 | 0.72 | 0.994515 |
Target: 5'- -cUGGAUACAgauccuGAAAUGCUGguGUAUCa -3' miRNA: 3'- auGCCUGUGU------UUUUAUGGCguUAUAG- -5' |
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27298 | 5' | -43.3 | NC_005856.1 | + | 26175 | 0.76 | 0.936234 |
Target: 5'- aACGGACuCGAAAAgACCGCcgGAUAUCc -3' miRNA: 3'- aUGCCUGuGUUUUUaUGGCG--UUAUAG- -5' |
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27298 | 5' | -43.3 | NC_005856.1 | + | 48367 | 1.11 | 0.02286 |
Target: 5'- uUACGGACACAAAAAUACCGCAAUAUCg -3' miRNA: 3'- -AUGCCUGUGUUUUUAUGGCGUUAUAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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