Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 27298 | 5' | -43.3 | NC_005856.1 | + | 89771 | 0.66 | 0.999986 |
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Target: 5'- gACuGGCACAGagGAAUugCGUaAAUGUCa -3' miRNA: 3'- aUGcCUGUGUU--UUUAugGCG-UUAUAG- -5' |
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| 27298 | 5' | -43.3 | NC_005856.1 | + | 80364 | 0.66 | 0.99999 |
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Target: 5'- gGCGGcCACGAccaacgcGCCGCAuauUGUCu -3' miRNA: 3'- aUGCCuGUGUUuuua---UGGCGUu--AUAG- -5' |
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| 27298 | 5' | -43.3 | NC_005856.1 | + | 75879 | 0.66 | 0.999993 |
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Target: 5'- -cCGGGCGCAAAGAUACaGCc----- -3' miRNA: 3'- auGCCUGUGUUUUUAUGgCGuuauag -5' |
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| 27298 | 5' | -43.3 | NC_005856.1 | + | 90439 | 0.66 | 0.999993 |
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Target: 5'- gUGCGGAUgugGCAu---UACCGUAAUGa- -3' miRNA: 3'- -AUGCCUG---UGUuuuuAUGGCGUUAUag -5' |
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| 27298 | 5' | -43.3 | NC_005856.1 | + | 11512 | 0.66 | 0.999993 |
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Target: 5'- aGCGGAUGCGAucgccaGAAUGCCGgAGa--- -3' miRNA: 3'- aUGCCUGUGUU------UUUAUGGCgUUauag -5' |
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| 27298 | 5' | -43.3 | NC_005856.1 | + | 23206 | 0.66 | 0.999993 |
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Target: 5'- uUACGGcagugagaGCAGAGAUAgCGCuGAUGUCc -3' miRNA: 3'- -AUGCCug------UGUUUUUAUgGCG-UUAUAG- -5' |
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| 27298 | 5' | -43.3 | NC_005856.1 | + | 77725 | 0.66 | 0.999993 |
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Target: 5'- gAUGG-CACAGGGAUcaaaGCCGCGAc--- -3' miRNA: 3'- aUGCCuGUGUUUUUA----UGGCGUUauag -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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