Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 27298 | 5' | -43.3 | NC_005856.1 | + | 30768 | 0.68 | 0.99987 |
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Target: 5'- uUGCGcGGCGCAAcggucagauauuGGAU-CCGCGAUGUUc -3' miRNA: 3'- -AUGC-CUGUGUU------------UUUAuGGCGUUAUAG- -5' |
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| 27298 | 5' | -43.3 | NC_005856.1 | + | 26175 | 0.76 | 0.936234 |
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Target: 5'- aACGGACuCGAAAAgACCGCcgGAUAUCc -3' miRNA: 3'- aUGCCUGuGUUUUUaUGGCG--UUAUAG- -5' |
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| 27298 | 5' | -43.3 | NC_005856.1 | + | 23206 | 0.66 | 0.999993 |
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Target: 5'- uUACGGcagugagaGCAGAGAUAgCGCuGAUGUCc -3' miRNA: 3'- -AUGCCug------UGUUUUUAUgGCG-UUAUAG- -5' |
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| 27298 | 5' | -43.3 | NC_005856.1 | + | 17706 | 0.7 | 0.999026 |
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Target: 5'- cGCGGugGCGAucGUGCUGUAAa--- -3' miRNA: 3'- aUGCCugUGUUuuUAUGGCGUUauag -5' |
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| 27298 | 5' | -43.3 | NC_005856.1 | + | 15019 | 0.71 | 0.997297 |
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Target: 5'- -uCGGugGCAAAGAUAgCGCGAc--- -3' miRNA: 3'- auGCCugUGUUUUUAUgGCGUUauag -5' |
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| 27298 | 5' | -43.3 | NC_005856.1 | + | 11512 | 0.66 | 0.999993 |
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Target: 5'- aGCGGAUGCGAucgccaGAAUGCCGgAGa--- -3' miRNA: 3'- aUGCCUGUGUU------UUUAUGGCgUUauag -5' |
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| 27298 | 5' | -43.3 | NC_005856.1 | + | 2201 | 0.66 | 0.99998 |
|
Target: 5'- aUGCgGGAUACucucg-ACCGCcGUAUCg -3' miRNA: 3'- -AUG-CCUGUGuuuuuaUGGCGuUAUAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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