Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 27299 | 5' | -51.3 | NC_005856.1 | + | 48798 | 1.06 | 0.005645 |
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Target: 5'- aUACAGGCAGUAUGCAAAGCGAUCGCUu -3' miRNA: 3'- -AUGUCCGUCAUACGUUUCGCUAGCGA- -5' |
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| 27299 | 5' | -51.3 | NC_005856.1 | + | 28827 | 0.73 | 0.625747 |
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Target: 5'- aUACAGGguGUGgacaugGCAGAuGCGAUCGg- -3' miRNA: 3'- -AUGUCCguCAUa-----CGUUU-CGCUAGCga -5' |
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| 27299 | 5' | -51.3 | NC_005856.1 | + | 2111 | 0.71 | 0.7251 |
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Target: 5'- aAguGGCAGaagGCAgccagagaacGAGCGAUCGCUc -3' miRNA: 3'- aUguCCGUCauaCGU----------UUCGCUAGCGA- -5' |
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| 27299 | 5' | -51.3 | NC_005856.1 | + | 32065 | 0.69 | 0.844075 |
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Target: 5'- aACAuGGCAGcaaAUGC--GGCGAUUGCUg -3' miRNA: 3'- aUGU-CCGUCa--UACGuuUCGCUAGCGA- -5' |
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| 27299 | 5' | -51.3 | NC_005856.1 | + | 11333 | 0.67 | 0.912992 |
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Target: 5'- aGCAGGCAaUGgacGCAgcAAGCGcgCGCa -3' miRNA: 3'- aUGUCCGUcAUa--CGU--UUCGCuaGCGa -5' |
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| 27299 | 5' | -51.3 | NC_005856.1 | + | 40555 | 0.67 | 0.925315 |
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Target: 5'- cUACAGGUgcuuucAGUAUGCcGAuGCGG-CGCUg -3' miRNA: 3'- -AUGUCCG------UCAUACGuUU-CGCUaGCGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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