miRNA display CGI


Results 1 - 6 of 6 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
27299 5' -51.3 NC_005856.1 + 2111 0.71 0.7251
Target:  5'- aAguGGCAGaagGCAgccagagaacGAGCGAUCGCUc -3'
miRNA:   3'- aUguCCGUCauaCGU----------UUCGCUAGCGA- -5'
27299 5' -51.3 NC_005856.1 + 11333 0.67 0.912992
Target:  5'- aGCAGGCAaUGgacGCAgcAAGCGcgCGCa -3'
miRNA:   3'- aUGUCCGUcAUa--CGU--UUCGCuaGCGa -5'
27299 5' -51.3 NC_005856.1 + 28827 0.73 0.625747
Target:  5'- aUACAGGguGUGgacaugGCAGAuGCGAUCGg- -3'
miRNA:   3'- -AUGUCCguCAUa-----CGUUU-CGCUAGCga -5'
27299 5' -51.3 NC_005856.1 + 32065 0.69 0.844075
Target:  5'- aACAuGGCAGcaaAUGC--GGCGAUUGCUg -3'
miRNA:   3'- aUGU-CCGUCa--UACGuuUCGCUAGCGA- -5'
27299 5' -51.3 NC_005856.1 + 40555 0.67 0.925315
Target:  5'- cUACAGGUgcuuucAGUAUGCcGAuGCGG-CGCUg -3'
miRNA:   3'- -AUGUCCG------UCAUACGuUU-CGCUaGCGA- -5'
27299 5' -51.3 NC_005856.1 + 48798 1.06 0.005645
Target:  5'- aUACAGGCAGUAUGCAAAGCGAUCGCUu -3'
miRNA:   3'- -AUGUCCGUCAUACGUUUCGCUAGCGA- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.