Results 1 - 11 of 11 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
27315 | 3' | -45.3 | NC_005856.1 | + | 15509 | 0.66 | 0.999911 |
Target: 5'- gCC-AGCACCCcuggcaUUGCGGUuucaccguaUGGCGGg -3' miRNA: 3'- -GGuUCGUGGGuaa---AACGUUA---------ACUGCU- -5' |
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27315 | 3' | -45.3 | NC_005856.1 | + | 35995 | 0.66 | 0.999845 |
Target: 5'- aCCu-GCGCCCAUUcagacCAGUUGGCa- -3' miRNA: 3'- -GGuuCGUGGGUAAaac--GUUAACUGcu -5' |
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27315 | 3' | -45.3 | NC_005856.1 | + | 32736 | 0.66 | 0.999845 |
Target: 5'- aCCu-GCGCCCAUUcagacCAGUUGGCa- -3' miRNA: 3'- -GGuuCGUGGGUAAaac--GUUAACUGcu -5' |
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27315 | 3' | -45.3 | NC_005856.1 | + | 78449 | 0.67 | 0.999439 |
Target: 5'- cCCaAAGCACCCGgcaauauccucugucGCGGUaUGACGAc -3' miRNA: 3'- -GG-UUCGUGGGUaaaa-----------CGUUA-ACUGCU- -5' |
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27315 | 3' | -45.3 | NC_005856.1 | + | 73639 | 0.67 | 0.999139 |
Target: 5'- cUCAAGCAUCUuUUccGCAAUUGcCGAc -3' miRNA: 3'- -GGUUCGUGGGuAAaaCGUUAACuGCU- -5' |
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27315 | 3' | -45.3 | NC_005856.1 | + | 67218 | 0.68 | 0.998387 |
Target: 5'- gCCAcugauugcAGCGCCa--UUUGCAAUaaUGGCGGa -3' miRNA: 3'- -GGU--------UCGUGGguaAAACGUUA--ACUGCU- -5' |
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27315 | 3' | -45.3 | NC_005856.1 | + | 21471 | 0.69 | 0.997625 |
Target: 5'- gCGAGCGCCgAUcaUUUGCAugGUgccgcccuggcUGACGAu -3' miRNA: 3'- gGUUCGUGGgUA--AAACGU--UA-----------ACUGCU- -5' |
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27315 | 3' | -45.3 | NC_005856.1 | + | 13317 | 0.69 | 0.996583 |
Target: 5'- aUAAGCGCCUggccagGUUUgGCA-UUGACGAa -3' miRNA: 3'- gGUUCGUGGG------UAAAaCGUuAACUGCU- -5' |
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27315 | 3' | -45.3 | NC_005856.1 | + | 8040 | 0.7 | 0.99434 |
Target: 5'- -gGAGCACCCAUUUcGCAucaucaaGCGAc -3' miRNA: 3'- ggUUCGUGGGUAAAaCGUuaac---UGCU- -5' |
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27315 | 3' | -45.3 | NC_005856.1 | + | 61971 | 0.71 | 0.986184 |
Target: 5'- -uGAGCGCCUGgcUUUGCAgucagcuGUUGGCGAg -3' miRNA: 3'- ggUUCGUGGGUa-AAACGU-------UAACUGCU- -5' |
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27315 | 3' | -45.3 | NC_005856.1 | + | 11553 | 1.13 | 0.01104 |
Target: 5'- aCCAAGCACCCAUUUUGCAAUUGACGAc -3' miRNA: 3'- -GGUUCGUGGGUAAAACGUUAACUGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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