Results 21 - 30 of 30 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 2732 | 3' | -57.9 | NC_001491.2 | + | 142856 | 0.66 | 0.852576 |
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Target: 5'- cGGuUUCGAUCCGuuacauuuucccaCGGGUACCGGg -3' miRNA: 3'- aCC-AGGCUAGGCuucg---------GCUCGUGGCC- -5' |
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| 2732 | 3' | -57.9 | NC_001491.2 | + | 144608 | 0.75 | 0.375417 |
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Target: 5'- aGGUCCGGggCGAAGUCGcuGCGCUGGc -3' miRNA: 3'- aCCAGGCUagGCUUCGGCu-CGUGGCC- -5' |
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| 2732 | 3' | -57.9 | NC_001491.2 | + | 144916 | 0.7 | 0.634424 |
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Target: 5'- cGG--CGAgccCCGGAGCCGGGCuggguGCCGGg -3' miRNA: 3'- aCCagGCUa--GGCUUCGGCUCG-----UGGCC- -5' |
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| 2732 | 3' | -57.9 | NC_001491.2 | + | 145419 | 0.75 | 0.39078 |
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Target: 5'- cGGgcgCCGAagCUGGAGCCGGagcggguGCGCCGGg -3' miRNA: 3'- aCCa--GGCUa-GGCUUCGGCU-------CGUGGCC- -5' |
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| 2732 | 3' | -57.9 | NC_001491.2 | + | 146433 | 0.66 | 0.854865 |
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Target: 5'- cGGaCCGAgggcgCCGggGCCucGGGCuguuGCUGGc -3' miRNA: 3'- aCCaGGCUa----GGCuuCGG--CUCG----UGGCC- -5' |
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| 2732 | 3' | -57.9 | NC_001491.2 | + | 146906 | 0.78 | 0.268954 |
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Target: 5'- cGGUCCaggCCGAGGcCCGGGC-CCGGg -3' miRNA: 3'- aCCAGGcuaGGCUUC-GGCUCGuGGCC- -5' |
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| 2732 | 3' | -57.9 | NC_001491.2 | + | 147068 | 0.69 | 0.703824 |
|
Target: 5'- gGGUCUGAaggcuccccUCCGcGGCCGcGGaGCCGGa -3' miRNA: 3'- aCCAGGCU---------AGGCuUCGGC-UCgUGGCC- -5' |
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| 2732 | 3' | -57.9 | NC_001491.2 | + | 147316 | 0.73 | 0.488179 |
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Target: 5'- -cGUCCGAggaGGAGCCGGGCAUCGc -3' miRNA: 3'- acCAGGCUaggCUUCGGCUCGUGGCc -5' |
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| 2732 | 3' | -57.9 | NC_001491.2 | + | 147390 | 0.68 | 0.770388 |
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Target: 5'- cUGGgccgCCGGcUCCaGggGCUcgGAGCGCCGc -3' miRNA: 3'- -ACCa---GGCU-AGG-CuuCGG--CUCGUGGCc -5' |
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| 2732 | 3' | -57.9 | NC_001491.2 | + | 147706 | 0.71 | 0.564901 |
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Target: 5'- gGGcCUGGggaUCGAAGgUGAGCGCCGGg -3' miRNA: 3'- aCCaGGCUa--GGCUUCgGCUCGUGGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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