Results 21 - 30 of 30 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 2732 | 3' | -57.9 | NC_001491.2 | + | 142856 | 0.66 | 0.852576 |
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Target: 5'- cGGuUUCGAUCCGuuacauuuucccaCGGGUACCGGg -3' miRNA: 3'- aCC-AGGCUAGGCuucg---------GCUCGUGGCC- -5' |
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| 2732 | 3' | -57.9 | NC_001491.2 | + | 15620 | 0.66 | 0.847168 |
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Target: 5'- gGGUCCc--UCGAAGCgGuGCGcCCGGa -3' miRNA: 3'- aCCAGGcuaGGCUUCGgCuCGU-GGCC- -5' |
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| 2732 | 3' | -57.9 | NC_001491.2 | + | 55674 | 0.66 | 0.847168 |
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Target: 5'- aGGUCCGAaUCGAcgcuCCGGGCgGCCGu -3' miRNA: 3'- aCCAGGCUaGGCUuc--GGCUCG-UGGCc -5' |
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| 2732 | 3' | -57.9 | NC_001491.2 | + | 126579 | 0.66 | 0.847168 |
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Target: 5'- aGGUCCucGUCCGcGGCC--GCACCGc -3' miRNA: 3'- aCCAGGc-UAGGCuUCGGcuCGUGGCc -5' |
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| 2732 | 3' | -57.9 | NC_001491.2 | + | 116697 | 0.67 | 0.82299 |
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Target: 5'- cGG-CCGcUCCGggGaCCaGGGCGCCc- -3' miRNA: 3'- aCCaGGCuAGGCuuC-GG-CUCGUGGcc -5' |
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| 2732 | 3' | -57.9 | NC_001491.2 | + | 89651 | 0.67 | 0.814591 |
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Target: 5'- aUGGUCCacaGUCguuucuugcgagCGggGuuGAGCGCuCGGg -3' miRNA: 3'- -ACCAGGc--UAG------------GCuuCggCUCGUG-GCC- -5' |
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| 2732 | 3' | -57.9 | NC_001491.2 | + | 140109 | 0.67 | 0.814591 |
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Target: 5'- cGGaCgCGcUCCGGAGC--GGCGCCGGg -3' miRNA: 3'- aCCaG-GCuAGGCUUCGgcUCGUGGCC- -5' |
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| 2732 | 3' | -57.9 | NC_001491.2 | + | 5681 | 0.67 | 0.788479 |
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Target: 5'- gUGG-CUGGgaaaagCCGGAGCCGGaaaacGUGCCGGc -3' miRNA: 3'- -ACCaGGCUa-----GGCUUCGGCU-----CGUGGCC- -5' |
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| 2732 | 3' | -57.9 | NC_001491.2 | + | 115838 | 0.68 | 0.770388 |
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Target: 5'- cGGcgcUCCGAgcccCUGGAGCCGgcGGCccagGCCGGa -3' miRNA: 3'- aCC---AGGCUa---GGCUUCGGC--UCG----UGGCC- -5' |
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| 2732 | 3' | -57.9 | NC_001491.2 | + | 146906 | 0.78 | 0.268954 |
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Target: 5'- cGGUCCaggCCGAGGcCCGGGC-CCGGg -3' miRNA: 3'- aCCAGGcuaGGCUUC-GGCUCGuGGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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