Results 1 - 20 of 52 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
27407 | 3' | -53.2 | NC_005869.1 | + | 1439 | 0.65 | 0.709906 |
Target: 5'- aCCCGGcc-GCCCuguuccaagggggcGGGGAG-UGCcuAGCCg -3' miRNA: 3'- -GGGUCuuuCGGG--------------UCCUUCaACG--UCGG- -5' |
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27407 | 3' | -53.2 | NC_005869.1 | + | 19390 | 0.66 | 0.701966 |
Target: 5'- gUCCGGGGAGaCCAgcagcgucuGGGAGgcGguGCCc -3' miRNA: 3'- -GGGUCUUUCgGGU---------CCUUCaaCguCGG- -5' |
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27407 | 3' | -53.2 | NC_005869.1 | + | 12343 | 0.66 | 0.701966 |
Target: 5'- gCgUAGAGgagguucuGGCCCAGGucGGUgagGguGCCc -3' miRNA: 3'- -GgGUCUU--------UCGGGUCCu-UCAa--CguCGG- -5' |
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27407 | 3' | -53.2 | NC_005869.1 | + | 9888 | 0.66 | 0.701966 |
Target: 5'- uCCCGGucGGCguCCAGGuGGGUgcgaccGUAGCCc -3' miRNA: 3'- -GGGUCuuUCG--GGUCC-UUCAa-----CGUCGG- -5' |
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27407 | 3' | -53.2 | NC_005869.1 | + | 2158 | 0.66 | 0.690561 |
Target: 5'- -gCAGuacAGCCUguguGGGggGUUGgAGCUg -3' miRNA: 3'- ggGUCuu-UCGGG----UCCuuCAACgUCGG- -5' |
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27407 | 3' | -53.2 | NC_005869.1 | + | 11801 | 0.66 | 0.690561 |
Target: 5'- cCCUGGAcGAGCCUuGGGAGcaggcucUGCGGCg -3' miRNA: 3'- -GGGUCU-UUCGGGuCCUUCa------ACGUCGg -5' |
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27407 | 3' | -53.2 | NC_005869.1 | + | 12943 | 0.66 | 0.690561 |
Target: 5'- uCCCGGAgcAGGaCgAGGggGaggaGCGGCCc -3' miRNA: 3'- -GGGUCU--UUCgGgUCCuuCaa--CGUCGG- -5' |
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27407 | 3' | -53.2 | NC_005869.1 | + | 26349 | 0.66 | 0.689416 |
Target: 5'- aCCCAGAccucgccGAGCUggcgCAGGAGcGggGCcccGGCCu -3' miRNA: 3'- -GGGUCU-------UUCGG----GUCCUU-CaaCG---UCGG- -5' |
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27407 | 3' | -53.2 | NC_005869.1 | + | 18778 | 0.66 | 0.679093 |
Target: 5'- gCCCAGuuGGCCCGGu-AGUgGC-GUCg -3' miRNA: 3'- -GGGUCuuUCGGGUCcuUCAaCGuCGG- -5' |
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27407 | 3' | -53.2 | NC_005869.1 | + | 5298 | 0.66 | 0.677943 |
Target: 5'- gCCGGAGGGCCCgcgugAGGGuggucucggugacGGUgaaggggGCGGUCu -3' miRNA: 3'- gGGUCUUUCGGG-----UCCU-------------UCAa------CGUCGG- -5' |
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27407 | 3' | -53.2 | NC_005869.1 | + | 7768 | 0.66 | 0.667576 |
Target: 5'- gCCGcAGGGCCUu-GAAGgcGCGGCCg -3' miRNA: 3'- gGGUcUUUCGGGucCUUCaaCGUCGG- -5' |
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27407 | 3' | -53.2 | NC_005869.1 | + | 13001 | 0.66 | 0.667576 |
Target: 5'- gCCgCGGAAggcGGCCC----AGUUGCGGCUg -3' miRNA: 3'- -GG-GUCUU---UCGGGuccuUCAACGUCGG- -5' |
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27407 | 3' | -53.2 | NC_005869.1 | + | 20906 | 0.66 | 0.667576 |
Target: 5'- uCCCAGAGAGgCCAGaGAGa-GCAGg- -3' miRNA: 3'- -GGGUCUUUCgGGUCcUUCaaCGUCgg -5' |
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27407 | 3' | -53.2 | NC_005869.1 | + | 20705 | 0.66 | 0.656022 |
Target: 5'- cUCCAGGGAGgagCGGGAGGcgcggcggcgGCGGCCg -3' miRNA: 3'- -GGGUCUUUCgg-GUCCUUCaa--------CGUCGG- -5' |
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27407 | 3' | -53.2 | NC_005869.1 | + | 26759 | 0.67 | 0.644443 |
Target: 5'- uUCCAGcGAuCuCCAGGAAGgccGCAGCa -3' miRNA: 3'- -GGGUCuUUcG-GGUCCUUCaa-CGUCGg -5' |
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27407 | 3' | -53.2 | NC_005869.1 | + | 27651 | 0.67 | 0.632852 |
Target: 5'- cUCCGGAccGCCCGGGAccuaccccgaccAGUU-CAucGCCa -3' miRNA: 3'- -GGGUCUuuCGGGUCCU------------UCAAcGU--CGG- -5' |
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27407 | 3' | -53.2 | NC_005869.1 | + | 21018 | 0.67 | 0.62126 |
Target: 5'- --aGGAGGGCUCugAGGAGGcUGCGGaCCg -3' miRNA: 3'- gggUCUUUCGGG--UCCUUCaACGUC-GG- -5' |
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27407 | 3' | -53.2 | NC_005869.1 | + | 10337 | 0.67 | 0.62126 |
Target: 5'- gCCCGGcgguGGCCCGGccgcuGcUGCAGCUg -3' miRNA: 3'- -GGGUCuu--UCGGGUCcuu--CaACGUCGG- -5' |
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27407 | 3' | -53.2 | NC_005869.1 | + | 21130 | 0.67 | 0.62126 |
Target: 5'- gCCGGugaaGAAGCCCuccGAGG--GCAGCCa -3' miRNA: 3'- gGGUC----UUUCGGGuc-CUUCaaCGUCGG- -5' |
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27407 | 3' | -53.2 | NC_005869.1 | + | 17332 | 0.67 | 0.614309 |
Target: 5'- uCCCAGggGGUgCAGGuGGcuuuccaaccagcgUGCuAGCCc -3' miRNA: 3'- -GGGUCuuUCGgGUCCuUCa-------------ACG-UCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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