miRNA: 3'- aACCGAUUuuagUCUGGUGg----GgCCCG- -5'
miRNA: 3'- aACCGauuuuaGUCUGGu---GGGGCCCG- -5'
miRNA: 3'- aaCCGAuuuuagUCUGGUGG-GgCCCG- -5'
miRNA: 3'- -AACCGAUUUUAGUCUGGUGGGGCCCG- -5'
Results 21 - 24 of 24 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 27407 | 5' | -54.1 | NC_005869.1 | + | 6956 | 0.68 | 0.506891 |
|
Target: 5'- gUGGCUGAua--AGACCACauguaCgGGGUg -3' miRNA: 3'- aACCGAUUuuagUCUGGUGg----GgCCCG- -5' |
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| 27407 | 5' | -54.1 | NC_005869.1 | + | 20628 | 0.66 | 0.574671 |
|
Target: 5'- cUGGCcacgggCAGGCCcaucUCCUGGGCg -3' miRNA: 3'- aACCGauuuuaGUCUGGu---GGGGCCCG- -5' |
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| 27407 | 5' | -54.1 | NC_005869.1 | + | 17461 | 0.66 | 0.597781 |
|
Target: 5'- -cGGCU------GGACCGCCgCgGGGCu -3' miRNA: 3'- aaCCGAuuuuagUCUGGUGG-GgCCCG- -5' |
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| 27407 | 5' | -54.1 | NC_005869.1 | + | 31558 | 1.09 | 0.000628 |
|
Target: 5'- gUUGGCUAAAAUCAGACCACCCCGGGCc -3' miRNA: 3'- -AACCGAUUUUAGUCUGGUGGGGCCCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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