Results 1 - 20 of 36 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
27420 | 3' | -68.4 | NC_005869.1 | + | 33862 | 0.66 | 0.137432 |
Target: 5'- cUCCgCCGcGaCCUGCGacuuugaCCCCG-CCCCu -3' miRNA: 3'- cAGG-GGC-C-GGACGCgg-----GGGGCaGGGG- -5' |
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27420 | 3' | -68.4 | NC_005869.1 | + | 15207 | 0.66 | 0.137432 |
Target: 5'- cUCCauguaCGGgg-GCGCCCgccgCCUGUCCCCg -3' miRNA: 3'- cAGGg----GCCggaCGCGGG----GGGCAGGGG- -5' |
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27420 | 3' | -68.4 | NC_005869.1 | + | 17280 | 0.66 | 0.136353 |
Target: 5'- -gCCCCGGCUgaggaGCGCCUCggugggcguccagaCCGcUCCCg -3' miRNA: 3'- caGGGGCCGGa----CGCGGGG--------------GGC-AGGGg -5' |
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27420 | 3' | -68.4 | NC_005869.1 | + | 23168 | 0.66 | 0.130382 |
Target: 5'- -cUCCCGGUccagCUGCaUCUCCCG-CCCCg -3' miRNA: 3'- caGGGGCCG----GACGcGGGGGGCaGGGG- -5' |
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27420 | 3' | -68.4 | NC_005869.1 | + | 17546 | 0.66 | 0.130039 |
Target: 5'- -aCCCCGGUggcgguggCaGCGCCgCCCGcccgcagcggcggUCCCCc -3' miRNA: 3'- caGGGGCCG--------GaCGCGGgGGGC-------------AGGGG- -5' |
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27420 | 3' | -68.4 | NC_005869.1 | + | 31939 | 0.66 | 0.126984 |
Target: 5'- -cCCCCcGCCU-C-CUCCCCGcUCCCCu -3' miRNA: 3'- caGGGGcCGGAcGcGGGGGGC-AGGGG- -5' |
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27420 | 3' | -68.4 | NC_005869.1 | + | 16436 | 0.66 | 0.123014 |
Target: 5'- uGUCCCCucgcacGGCCgucaagcgccggGCGCCCgCCGacgagCCCa -3' miRNA: 3'- -CAGGGG------CCGGa-----------CGCGGGgGGCa----GGGg -5' |
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27420 | 3' | -68.4 | NC_005869.1 | + | 9740 | 0.66 | 0.120432 |
Target: 5'- aGUCCUCcuCCUGCuCCuCCUCGUCCCg -3' miRNA: 3'- -CAGGGGccGGACGcGG-GGGGCAGGGg -5' |
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27420 | 3' | -68.4 | NC_005869.1 | + | 9293 | 0.66 | 0.117275 |
Target: 5'- gGUCCUgguUGGCgCagGCcgGCUCCUCGUCCCCc -3' miRNA: 3'- -CAGGG---GCCG-Ga-CG--CGGGGGGCAGGGG- -5' |
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27420 | 3' | -68.4 | NC_005869.1 | + | 17105 | 0.66 | 0.117275 |
Target: 5'- aGUCCU--GCagGCGCCCCCCG-CCCg -3' miRNA: 3'- -CAGGGgcCGgaCGCGGGGGGCaGGGg -5' |
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27420 | 3' | -68.4 | NC_005869.1 | + | 32010 | 0.67 | 0.114196 |
Target: 5'- -cCUUCGaGCCUGCGCCCgCUCGcuuccaUCCCa -3' miRNA: 3'- caGGGGC-CGGACGCGGG-GGGC------AGGGg -5' |
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27420 | 3' | -68.4 | NC_005869.1 | + | 31410 | 0.67 | 0.114196 |
Target: 5'- cUCCCCcGCCgcUGCGCCCCgauggccuCCG-CCgCCa -3' miRNA: 3'- cAGGGGcCGG--ACGCGGGG--------GGCaGG-GG- -5' |
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27420 | 3' | -68.4 | NC_005869.1 | + | 17013 | 0.67 | 0.108262 |
Target: 5'- -aUCCCGGCCagGCaGCCCCgCaCGguaUCCCUg -3' miRNA: 3'- caGGGGCCGGa-CG-CGGGG-G-GC---AGGGG- -5' |
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27420 | 3' | -68.4 | NC_005869.1 | + | 25653 | 0.67 | 0.107685 |
Target: 5'- -gCCCUGGCCaaccucuuccagcUGCaccuccugcgcccGCCCCCCGaCCUCc -3' miRNA: 3'- caGGGGCCGG-------------ACG-------------CGGGGGGCaGGGG- -5' |
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27420 | 3' | -68.4 | NC_005869.1 | + | 29077 | 0.67 | 0.102619 |
Target: 5'- -aCCCCGGCUgGCaCCUgCCGcUCCCUc -3' miRNA: 3'- caGGGGCCGGaCGcGGGgGGC-AGGGG- -5' |
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27420 | 3' | -68.4 | NC_005869.1 | + | 25812 | 0.67 | 0.102344 |
Target: 5'- -cCCCCgcccgagGGCCcGcCGCCCCgCC-UCCCCu -3' miRNA: 3'- caGGGG-------CCGGaC-GCGGGG-GGcAGGGG- -5' |
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27420 | 3' | -68.4 | NC_005869.1 | + | 28953 | 0.67 | 0.099902 |
Target: 5'- -gCCCCucaaGGCCUuCGCCUCCC-UCuCCCa -3' miRNA: 3'- caGGGG----CCGGAcGCGGGGGGcAG-GGG- -5' |
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27420 | 3' | -68.4 | NC_005869.1 | + | 21081 | 0.67 | 0.097253 |
Target: 5'- cGUCCCagGGCC-GCuccucCCCCUCGUCCUg -3' miRNA: 3'- -CAGGGg-CCGGaCGc----GGGGGGCAGGGg -5' |
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27420 | 3' | -68.4 | NC_005869.1 | + | 7363 | 0.68 | 0.094671 |
Target: 5'- gGUCCUCgggGGCCaUGaCGCCCUCC-UCCCa -3' miRNA: 3'- -CAGGGG---CCGG-AC-GCGGGGGGcAGGGg -5' |
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27420 | 3' | -68.4 | NC_005869.1 | + | 21097 | 0.68 | 0.092154 |
Target: 5'- -gCCCUGGgCU-CGCCCUUCGaCCCCu -3' miRNA: 3'- caGGGGCCgGAcGCGGGGGGCaGGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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