Results 1 - 20 of 28 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
27556 | 3' | -59.9 | NC_005882.1 | + | 9546 | 0.66 | 0.366529 |
Target: 5'- cGCcAGCGCGccaaucgcagCGGCuuucaccucaagcGACGCGGACCGGu -3' miRNA: 3'- -UGcUCGCGCa---------GCCG-------------CUGUGCCUGGUU- -5' |
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27556 | 3' | -59.9 | NC_005882.1 | + | 34482 | 0.66 | 0.35879 |
Target: 5'- aGCGGGC-CGUCGGCucgguCGuCGGAUCGAu -3' miRNA: 3'- -UGCUCGcGCAGCCGcu---GU-GCCUGGUU- -5' |
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27556 | 3' | -59.9 | NC_005882.1 | + | 12319 | 0.66 | 0.342004 |
Target: 5'- cGCGAucaGCGCGUgacggcugcCGGCGGCGCcGAUCGg -3' miRNA: 3'- -UGCU---CGCGCA---------GCCGCUGUGcCUGGUu -5' |
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27556 | 3' | -59.9 | NC_005882.1 | + | 22783 | 0.66 | 0.342004 |
Target: 5'- aACGAGCcCGUCacguuugacGCGGCaACGGGCCGu -3' miRNA: 3'- -UGCUCGcGCAGc--------CGCUG-UGCCUGGUu -5' |
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27556 | 3' | -59.9 | NC_005882.1 | + | 26946 | 0.67 | 0.317903 |
Target: 5'- gGCGGGCGCGgcCGGCaaguuCGCGG-CCGu -3' miRNA: 3'- -UGCUCGCGCa-GCCGcu---GUGCCuGGUu -5' |
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27556 | 3' | -59.9 | NC_005882.1 | + | 15278 | 0.67 | 0.317903 |
Target: 5'- uACGAGCggGCGUCGaccgggagcgccGUGAgcaGCGGGCCGAg -3' miRNA: 3'- -UGCUCG--CGCAGC------------CGCUg--UGCCUGGUU- -5' |
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27556 | 3' | -59.9 | NC_005882.1 | + | 12039 | 0.67 | 0.302559 |
Target: 5'- uGCGuuGCGCGUCaGCucGGCGCGGuacGCCAGc -3' miRNA: 3'- -UGCu-CGCGCAGcCG--CUGUGCC---UGGUU- -5' |
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27556 | 3' | -59.9 | NC_005882.1 | + | 19603 | 0.67 | 0.301807 |
Target: 5'- cGCGccuGCGCGUCGGCGagcugcugcuugaGCugGGugUu- -3' miRNA: 3'- -UGCu--CGCGCAGCCGC-------------UGugCCugGuu -5' |
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27556 | 3' | -59.9 | NC_005882.1 | + | 3260 | 0.67 | 0.287791 |
Target: 5'- cGCGAGCGUGcgggCGGCGuCACuGuCCAGc -3' miRNA: 3'- -UGCUCGCGCa---GCCGCuGUGcCuGGUU- -5' |
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27556 | 3' | -59.9 | NC_005882.1 | + | 23425 | 0.67 | 0.287791 |
Target: 5'- aGC-AGCuCGUCGGCGuCACGGGCg-- -3' miRNA: 3'- -UGcUCGcGCAGCCGCuGUGCCUGguu -5' |
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27556 | 3' | -59.9 | NC_005882.1 | + | 23236 | 0.67 | 0.287791 |
Target: 5'- cCGGGCGCGggcCGGCGgGCAC-GAUCAAc -3' miRNA: 3'- uGCUCGCGCa--GCCGC-UGUGcCUGGUU- -5' |
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27556 | 3' | -59.9 | NC_005882.1 | + | 12237 | 0.68 | 0.280622 |
Target: 5'- -gGAGCGCaucaCGGCGGC-CGGugCAu -3' miRNA: 3'- ugCUCGCGca--GCCGCUGuGCCugGUu -5' |
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27556 | 3' | -59.9 | NC_005882.1 | + | 17153 | 0.68 | 0.273596 |
Target: 5'- aGCGccuGCGCGUCGGCauucaggucgcGCGCGGACa-- -3' miRNA: 3'- -UGCu--CGCGCAGCCGc----------UGUGCCUGguu -5' |
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27556 | 3' | -59.9 | NC_005882.1 | + | 18284 | 0.68 | 0.266031 |
Target: 5'- gACGAuucacauGCGCGUCGGCGGCAagaaGGcguaCAAg -3' miRNA: 3'- -UGCU-------CGCGCAGCCGCUGUg---CCug--GUU- -5' |
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27556 | 3' | -59.9 | NC_005882.1 | + | 29736 | 0.68 | 0.246898 |
Target: 5'- gACGAGCgGCGacacgcugCGGCuuuccGCACGGGCCGg -3' miRNA: 3'- -UGCUCG-CGCa-------GCCGc----UGUGCCUGGUu -5' |
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27556 | 3' | -59.9 | NC_005882.1 | + | 1768 | 0.69 | 0.230727 |
Target: 5'- gACGGGauucggcguauucaGCGUgGGCG-CGCGGGCCGc -3' miRNA: 3'- -UGCUCg-------------CGCAgCCGCuGUGCCUGGUu -5' |
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27556 | 3' | -59.9 | NC_005882.1 | + | 21742 | 0.69 | 0.22832 |
Target: 5'- cGCGcGCGcCGUCGccaguuCGACGCGGACCu- -3' miRNA: 3'- -UGCuCGC-GCAGCc-----GCUGUGCCUGGuu -5' |
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27556 | 3' | -59.9 | NC_005882.1 | + | 22739 | 0.69 | 0.224749 |
Target: 5'- uCGAGCGCuugcgggaucgugaaGcCGGCGAgcUGCGGGCCGAa -3' miRNA: 3'- uGCUCGCG---------------CaGCCGCU--GUGCCUGGUU- -5' |
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27556 | 3' | -59.9 | NC_005882.1 | + | 31963 | 0.69 | 0.216601 |
Target: 5'- gACGA--GCGUCGGCGGcCGCuGACCGAu -3' miRNA: 3'- -UGCUcgCGCAGCCGCU-GUGcCUGGUU- -5' |
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27556 | 3' | -59.9 | NC_005882.1 | + | 3221 | 0.69 | 0.216601 |
Target: 5'- cGCGcAGCaCGUCGGaCGACuucgcgagguACGGGCCGAu -3' miRNA: 3'- -UGC-UCGcGCAGCC-GCUG----------UGCCUGGUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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