Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 27556 | 3' | -59.9 | NC_005882.1 | + | 31370 | 0.7 | 0.205402 |
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Target: 5'- gGCGAGCuG-GUCGGCGugACGcGGCUg- -3' miRNA: 3'- -UGCUCG-CgCAGCCGCugUGC-CUGGuu -5' |
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| 27556 | 3' | -59.9 | NC_005882.1 | + | 27557 | 0.7 | 0.194707 |
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Target: 5'- uCGGGCGCucGUCGGCGgucgccgcGCGCGcGGCCGc -3' miRNA: 3'- uGCUCGCG--CAGCCGC--------UGUGC-CUGGUu -5' |
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| 27556 | 3' | -59.9 | NC_005882.1 | + | 22171 | 0.7 | 0.184005 |
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Target: 5'- gGCGGGCGCcgugauuGUCGaaGACGCGGAgCCGAa -3' miRNA: 3'- -UGCUCGCG-------CAGCcgCUGUGCCU-GGUU- -5' |
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| 27556 | 3' | -59.9 | NC_005882.1 | + | 12716 | 0.71 | 0.170082 |
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Target: 5'- gACGcAGCGCG-CGGCGGCGCucGCCGg -3' miRNA: 3'- -UGC-UCGCGCaGCCGCUGUGccUGGUu -5' |
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| 27556 | 3' | -59.9 | NC_005882.1 | + | 19254 | 0.73 | 0.115356 |
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Target: 5'- aACGAagGCGuCGUCGaguucaGCGACugGGGCCAAg -3' miRNA: 3'- -UGCU--CGC-GCAGC------CGCUGugCCUGGUU- -5' |
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| 27556 | 3' | -59.9 | NC_005882.1 | + | 19806 | 0.75 | 0.08684 |
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Target: 5'- cGCGAGCGCGcCGGCGugACGacGACgGg -3' miRNA: 3'- -UGCUCGCGCaGCCGCugUGC--CUGgUu -5' |
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| 27556 | 3' | -59.9 | NC_005882.1 | + | 25470 | 0.8 | 0.037393 |
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Target: 5'- cGCGcAGCuCGUCGGCGACGCGG-CCGAg -3' miRNA: 3'- -UGC-UCGcGCAGCCGCUGUGCCuGGUU- -5' |
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| 27556 | 3' | -59.9 | NC_005882.1 | + | 2024 | 1.05 | 0.000414 |
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Target: 5'- uACGAGCGCGUCGGCGACACGGACCAAu -3' miRNA: 3'- -UGCUCGCGCAGCCGCUGUGCCUGGUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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