Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 27556 | 3' | -59.9 | NC_005882.1 | + | 23425 | 0.67 | 0.287791 |
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Target: 5'- aGC-AGCuCGUCGGCGuCACGGGCg-- -3' miRNA: 3'- -UGcUCGcGCAGCCGCuGUGCCUGguu -5' |
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| 27556 | 3' | -59.9 | NC_005882.1 | + | 25470 | 0.8 | 0.037393 |
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Target: 5'- cGCGcAGCuCGUCGGCGACGCGG-CCGAg -3' miRNA: 3'- -UGC-UCGcGCAGCCGCUGUGCCuGGUU- -5' |
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| 27556 | 3' | -59.9 | NC_005882.1 | + | 26946 | 0.67 | 0.317903 |
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Target: 5'- gGCGGGCGCGgcCGGCaaguuCGCGG-CCGu -3' miRNA: 3'- -UGCUCGCGCa-GCCGcu---GUGCCuGGUu -5' |
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| 27556 | 3' | -59.9 | NC_005882.1 | + | 27557 | 0.7 | 0.194707 |
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Target: 5'- uCGGGCGCucGUCGGCGgucgccgcGCGCGcGGCCGc -3' miRNA: 3'- uGCUCGCG--CAGCCGC--------UGUGC-CUGGUu -5' |
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| 27556 | 3' | -59.9 | NC_005882.1 | + | 29736 | 0.68 | 0.246898 |
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Target: 5'- gACGAGCgGCGacacgcugCGGCuuuccGCACGGGCCGg -3' miRNA: 3'- -UGCUCG-CGCa-------GCCGc----UGUGCCUGGUu -5' |
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| 27556 | 3' | -59.9 | NC_005882.1 | + | 31370 | 0.7 | 0.205402 |
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Target: 5'- gGCGAGCuG-GUCGGCGugACGcGGCUg- -3' miRNA: 3'- -UGCUCG-CgCAGCCGCugUGC-CUGGuu -5' |
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| 27556 | 3' | -59.9 | NC_005882.1 | + | 31963 | 0.69 | 0.216601 |
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Target: 5'- gACGA--GCGUCGGCGGcCGCuGACCGAu -3' miRNA: 3'- -UGCUcgCGCAGCCGCU-GUGcCUGGUU- -5' |
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| 27556 | 3' | -59.9 | NC_005882.1 | + | 34482 | 0.66 | 0.35879 |
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Target: 5'- aGCGGGC-CGUCGGCucgguCGuCGGAUCGAu -3' miRNA: 3'- -UGCUCGcGCAGCCGcu---GU-GCCUGGUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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