Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 27556 | 3' | -59.9 | NC_005882.1 | + | 19603 | 0.67 | 0.301807 |
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Target: 5'- cGCGccuGCGCGUCGGCGagcugcugcuugaGCugGGugUu- -3' miRNA: 3'- -UGCu--CGCGCAGCCGC-------------UGugCCugGuu -5' |
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| 27556 | 3' | -59.9 | NC_005882.1 | + | 12039 | 0.67 | 0.302559 |
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Target: 5'- uGCGuuGCGCGUCaGCucGGCGCGGuacGCCAGc -3' miRNA: 3'- -UGCu-CGCGCAGcCG--CUGUGCC---UGGUU- -5' |
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| 27556 | 3' | -59.9 | NC_005882.1 | + | 15278 | 0.67 | 0.317903 |
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Target: 5'- uACGAGCggGCGUCGaccgggagcgccGUGAgcaGCGGGCCGAg -3' miRNA: 3'- -UGCUCG--CGCAGC------------CGCUg--UGCCUGGUU- -5' |
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| 27556 | 3' | -59.9 | NC_005882.1 | + | 26946 | 0.67 | 0.317903 |
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Target: 5'- gGCGGGCGCGgcCGGCaaguuCGCGG-CCGu -3' miRNA: 3'- -UGCUCGCGCa-GCCGcu---GUGCCuGGUu -5' |
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| 27556 | 3' | -59.9 | NC_005882.1 | + | 12319 | 0.66 | 0.342004 |
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Target: 5'- cGCGAucaGCGCGUgacggcugcCGGCGGCGCcGAUCGg -3' miRNA: 3'- -UGCU---CGCGCA---------GCCGCUGUGcCUGGUu -5' |
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| 27556 | 3' | -59.9 | NC_005882.1 | + | 22783 | 0.66 | 0.342004 |
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Target: 5'- aACGAGCcCGUCacguuugacGCGGCaACGGGCCGu -3' miRNA: 3'- -UGCUCGcGCAGc--------CGCUG-UGCCUGGUu -5' |
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| 27556 | 3' | -59.9 | NC_005882.1 | + | 34482 | 0.66 | 0.35879 |
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Target: 5'- aGCGGGC-CGUCGGCucgguCGuCGGAUCGAu -3' miRNA: 3'- -UGCUCGcGCAGCCGcu---GU-GCCUGGUU- -5' |
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| 27556 | 3' | -59.9 | NC_005882.1 | + | 9546 | 0.66 | 0.366529 |
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Target: 5'- cGCcAGCGCGccaaucgcagCGGCuuucaccucaagcGACGCGGACCGGu -3' miRNA: 3'- -UGcUCGCGCa---------GCCG-------------CUGUGCCUGGUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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