Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 27560 | 3' | -56.7 | NC_005882.1 | + | 26611 | 0.68 | 0.377225 |
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Target: 5'- cGCGCgGCGCACguaGUCCugguCGAUGGu -3' miRNA: 3'- -CGCGaCGCGUGagcCAGGu---GCUACUg -5' |
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| 27560 | 3' | -56.7 | NC_005882.1 | + | 28052 | 0.7 | 0.317859 |
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Target: 5'- -aGCUGCGCGCggCGGUgagcgcaaaaCagGCGAUGGCg -3' miRNA: 3'- cgCGACGCGUGa-GCCA----------Gg-UGCUACUG- -5' |
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| 27560 | 3' | -56.7 | NC_005882.1 | + | 28272 | 0.66 | 0.512878 |
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Target: 5'- gGCgGCcGCGCAggCGGUCUccagcgucacaucgGCGGUGGCc -3' miRNA: 3'- -CG-CGaCGCGUgaGCCAGG--------------UGCUACUG- -5' |
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| 27560 | 3' | -56.7 | NC_005882.1 | + | 29154 | 0.66 | 0.50542 |
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Target: 5'- cGCGCUGaCGCugaagaUCGG-CUACGccGGCg -3' miRNA: 3'- -CGCGAC-GCGug----AGCCaGGUGCuaCUG- -5' |
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| 27560 | 3' | -56.7 | NC_005882.1 | + | 29992 | 0.66 | 0.526846 |
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Target: 5'- aGCGCUcgGCGCUCGccGUCCAgGccGGCa -3' miRNA: 3'- -CGCGAcgCGUGAGC--CAGGUgCuaCUG- -5' |
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| 27560 | 3' | -56.7 | NC_005882.1 | + | 30524 | 0.66 | 0.494847 |
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Target: 5'- aUGCUGCGCg--CGG-CCGacaaGGUGACg -3' miRNA: 3'- cGCGACGCGugaGCCaGGUg---CUACUG- -5' |
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| 27560 | 3' | -56.7 | NC_005882.1 | + | 31099 | 0.66 | 0.526846 |
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Target: 5'- gGCGCcuugcugacgGCGuCAUUCgugagacggaGGUgCCGCGAUGACg -3' miRNA: 3'- -CGCGa---------CGC-GUGAG----------CCA-GGUGCUACUG- -5' |
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| 27560 | 3' | -56.7 | NC_005882.1 | + | 33077 | 0.7 | 0.30224 |
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Target: 5'- aUGCUGCGCAagccCGGUCUGCGAcuGCu -3' miRNA: 3'- cGCGACGCGUga--GCCAGGUGCUacUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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