Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 27560 | 3' | -56.7 | NC_005882.1 | + | 26611 | 0.68 | 0.377225 |
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Target: 5'- cGCGCgGCGCACguaGUCCugguCGAUGGu -3' miRNA: 3'- -CGCGaCGCGUGagcCAGGu---GCUACUg -5' |
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| 27560 | 3' | -56.7 | NC_005882.1 | + | 3372 | 0.69 | 0.342411 |
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Target: 5'- gGCGCUGCGCGgcCUgGGcuaCCGCGugcUGGCc -3' miRNA: 3'- -CGCGACGCGU--GAgCCa--GGUGCu--ACUG- -5' |
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| 27560 | 3' | -56.7 | NC_005882.1 | + | 17153 | 0.69 | 0.342411 |
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Target: 5'- aGCGcCUGCGCgucggcAUUCaGGUCgCGCGcgGACa -3' miRNA: 3'- -CGC-GACGCG------UGAG-CCAG-GUGCuaCUG- -5' |
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| 27560 | 3' | -56.7 | NC_005882.1 | + | 33077 | 0.7 | 0.30224 |
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Target: 5'- aUGCUGCGCAagccCGGUCUGCGAcuGCu -3' miRNA: 3'- cGCGACGCGUga--GCCAGGUGCUacUG- -5' |
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| 27560 | 3' | -56.7 | NC_005882.1 | + | 2127 | 0.71 | 0.2658 |
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Target: 5'- cGCGCUGCGCcgaUUGGacaucaccgaUCCGCGcUGGCu -3' miRNA: 3'- -CGCGACGCGug-AGCC----------AGGUGCuACUG- -5' |
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| 27560 | 3' | -56.7 | NC_005882.1 | + | 1797 | 0.73 | 0.182566 |
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Target: 5'- uCGCUGCGCAa--GGgcgaCACGGUGACg -3' miRNA: 3'- cGCGACGCGUgagCCag--GUGCUACUG- -5' |
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| 27560 | 3' | -56.7 | NC_005882.1 | + | 3655 | 0.97 | 0.003178 |
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Target: 5'- cGCGCUGCGCACUCGGcUCACGAUGACc -3' miRNA: 3'- -CGCGACGCGUGAGCCaGGUGCUACUG- -5' |
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| 27560 | 3' | -56.7 | NC_005882.1 | + | 18023 | 0.66 | 0.537687 |
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Target: 5'- uCGCgGCGCgugACUgGGUCgACGGUG-Cg -3' miRNA: 3'- cGCGaCGCG---UGAgCCAGgUGCUACuG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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