Results 21 - 26 of 26 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
27562 | 5' | -59.3 | NC_005882.1 | + | 22968 | 0.7 | 0.218001 |
Target: 5'- gCGGCCaaGGCGACCUaCGACUacGCGGa -3' miRNA: 3'- -GUCGGagCUGCUGGA-GCUGGa-CGCCc -5' |
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27562 | 5' | -59.3 | NC_005882.1 | + | 24526 | 0.66 | 0.402026 |
Target: 5'- gGGCCaaGACGACaaCGACUgcgcaggUGUGGGg -3' miRNA: 3'- gUCGGagCUGCUGgaGCUGG-------ACGCCC- -5' |
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27562 | 5' | -59.3 | NC_005882.1 | + | 28450 | 0.72 | 0.176353 |
Target: 5'- uCAGCUUCugGACGACCUUGACC-GCGu- -3' miRNA: 3'- -GUCGGAG--CUGCUGGAGCUGGaCGCcc -5' |
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27562 | 5' | -59.3 | NC_005882.1 | + | 29226 | 0.68 | 0.334293 |
Target: 5'- uGGCUUCGACGaaccGCCgUCGACCgugacaauccGCGGa -3' miRNA: 3'- gUCGGAGCUGC----UGG-AGCUGGa---------CGCCc -5' |
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27562 | 5' | -59.3 | NC_005882.1 | + | 32181 | 0.67 | 0.342388 |
Target: 5'- gGGCgUguACGACgUUGACCUGcCGGGa -3' miRNA: 3'- gUCGgAgcUGCUGgAGCUGGAC-GCCC- -5' |
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27562 | 5' | -59.3 | NC_005882.1 | + | 33068 | 0.67 | 0.342388 |
Target: 5'- aAGCC-CGgucuGCGACugCUCGACCUGCGc- -3' miRNA: 3'- gUCGGaGC----UGCUG--GAGCUGGACGCcc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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