Results 21 - 26 of 26 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
27562 | 5' | -59.3 | NC_005882.1 | + | 2191 | 0.66 | 0.421441 |
Target: 5'- aGGCaCUCGuCGAUCaUCGACUUcGCGGc -3' miRNA: 3'- gUCG-GAGCuGCUGG-AGCUGGA-CGCCc -5' |
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27562 | 5' | -59.3 | NC_005882.1 | + | 11668 | 0.66 | 0.434695 |
Target: 5'- uCAGCCUCG-CGGCCgUCgucgcguacuggcucGAUC-GCGGGc -3' miRNA: 3'- -GUCGGAGCuGCUGG-AG---------------CUGGaCGCCC- -5' |
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27562 | 5' | -59.3 | NC_005882.1 | + | 18403 | 0.66 | 0.440448 |
Target: 5'- cCGGCCUUG-CGACgCgacgCGGCC-GCGGu -3' miRNA: 3'- -GUCGGAGCuGCUG-Ga---GCUGGaCGCCc -5' |
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27562 | 5' | -59.3 | NC_005882.1 | + | 16105 | 0.66 | 0.440448 |
Target: 5'- -cGUCUUcGCgGACCUCGACCUGUcucGGa -3' miRNA: 3'- guCGGAGcUG-CUGGAGCUGGACG---CCc -5' |
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27562 | 5' | -59.3 | NC_005882.1 | + | 14955 | 0.66 | 0.440448 |
Target: 5'- aAGCCUgGACGGCCgaggUGaaGCCaaGUGGGu -3' miRNA: 3'- gUCGGAgCUGCUGGa---GC--UGGa-CGCCC- -5' |
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27562 | 5' | -59.3 | NC_005882.1 | + | 11845 | 0.66 | 0.440448 |
Target: 5'- gUAGcCCUUGGCGACUcgauagUCGGCUU-CGGGc -3' miRNA: 3'- -GUC-GGAGCUGCUGG------AGCUGGAcGCCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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