Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
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P value |
| Predicted miRNA align pattern | |||||||
| 27564 | 5' | -55.2 | NC_005882.1 | + | 9254 | 0.66 | 0.629168 |
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Target: 5'- aGCGGUugGGGuauagccAgACGGCaUUCGggGCGc -3' miRNA: 3'- -CGCCGugCCU-------UgUGCUG-GAGCaaCGC- -5' |
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| 27564 | 5' | -55.2 | NC_005882.1 | + | 15008 | 0.66 | 0.619085 |
|
Target: 5'- aCGGCGCGGAugcugaggaagaGCugGACUgCG-UGCc -3' miRNA: 3'- cGCCGUGCCU------------UGugCUGGaGCaACGc -5' |
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| 27564 | 5' | -55.2 | NC_005882.1 | + | 12828 | 0.66 | 0.615726 |
|
Target: 5'- cCGGCGCcGGAUGCGccucuucaggauuuGCCUCGcUGCGu -3' miRNA: 3'- cGCCGUGcCUUGUGC--------------UGGAGCaACGC- -5' |
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| 27564 | 5' | -55.2 | NC_005882.1 | + | 13276 | 0.66 | 0.607894 |
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Target: 5'- aCGGCGCgccuGGAGCGCGAUUUUc-UGCGa -3' miRNA: 3'- cGCCGUG----CCUUGUGCUGGAGcaACGC- -5' |
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| 27564 | 5' | -55.2 | NC_005882.1 | + | 5343 | 0.66 | 0.619085 |
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Target: 5'- cGUGGCGaGGAAUACGACgugUCGUcggUGCc -3' miRNA: 3'- -CGCCGUgCCUUGUGCUGg--AGCA---ACGc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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