Results 1 - 20 of 32 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
27571 | 3' | -56.6 | NC_005882.1 | + | 25587 | 0.66 | 0.523566 |
Target: 5'- gGCGCCGCGcugCGuGaGCAAcgccagggGCUCGGCg -3' miRNA: 3'- -UGCGGCGUca-GC-C-CGUUaa------CGAGCUG- -5' |
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27571 | 3' | -56.6 | NC_005882.1 | + | 11705 | 0.66 | 0.522479 |
Target: 5'- gACGagaCuCAGUuuguagagggcgaCGGGCAGUUGCUgCGGCg -3' miRNA: 3'- -UGCg--GcGUCA-------------GCCCGUUAACGA-GCUG- -5' |
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27571 | 3' | -56.6 | NC_005882.1 | + | 21465 | 0.66 | 0.512735 |
Target: 5'- uGCGCCGUAGUCGguGGUcGUgucGUUCGuCa -3' miRNA: 3'- -UGCGGCGUCAGC--CCGuUAa--CGAGCuG- -5' |
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27571 | 3' | -56.6 | NC_005882.1 | + | 14773 | 0.66 | 0.501999 |
Target: 5'- gGCGCUGCugauGUgGGGCGGUcagGUucacaucauuUCGACg -3' miRNA: 3'- -UGCGGCGu---CAgCCCGUUAa--CG----------AGCUG- -5' |
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27571 | 3' | -56.6 | NC_005882.1 | + | 27541 | 0.66 | 0.470409 |
Target: 5'- gGCGCCGCAaucggcaUCGGGC----GCUCGuCg -3' miRNA: 3'- -UGCGGCGUc------AGCCCGuuaaCGAGCuG- -5' |
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27571 | 3' | -56.6 | NC_005882.1 | + | 22694 | 0.67 | 0.460105 |
Target: 5'- uGCGCCGCGucgacGUCGGacaGCGAcUGCacCGGCg -3' miRNA: 3'- -UGCGGCGU-----CAGCC---CGUUaACGa-GCUG- -5' |
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27571 | 3' | -56.6 | NC_005882.1 | + | 22476 | 0.67 | 0.460105 |
Target: 5'- cACGCUGgGGcgcgCGGGC--UUGgUCGACg -3' miRNA: 3'- -UGCGGCgUCa---GCCCGuuAACgAGCUG- -5' |
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27571 | 3' | -56.6 | NC_005882.1 | + | 30641 | 0.67 | 0.460105 |
Target: 5'- gGCGCUGCGcuGaCGGGCAAcgUGaacgUCGACg -3' miRNA: 3'- -UGCGGCGU--CaGCCCGUUa-ACg---AGCUG- -5' |
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27571 | 3' | -56.6 | NC_005882.1 | + | 23455 | 0.67 | 0.460105 |
Target: 5'- -gGCCGCuGUC-GGCGAUga-UCGACg -3' miRNA: 3'- ugCGGCGuCAGcCCGUUAacgAGCUG- -5' |
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27571 | 3' | -56.6 | NC_005882.1 | + | 21212 | 0.67 | 0.449921 |
Target: 5'- gACGCCGagcaGGUUGaccGGCGcUUGCUCGcCg -3' miRNA: 3'- -UGCGGCg---UCAGC---CCGUuAACGAGCuG- -5' |
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27571 | 3' | -56.6 | NC_005882.1 | + | 26121 | 0.67 | 0.449921 |
Target: 5'- aACGCCGCGGUCauuuccGGCAGagcCUUGGCc -3' miRNA: 3'- -UGCGGCGUCAGc-----CCGUUaacGAGCUG- -5' |
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27571 | 3' | -56.6 | NC_005882.1 | + | 30983 | 0.67 | 0.439863 |
Target: 5'- cACGCCGCAuGUUGuGcGCGAgucGgUCGACg -3' miRNA: 3'- -UGCGGCGU-CAGC-C-CGUUaa-CgAGCUG- -5' |
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27571 | 3' | -56.6 | NC_005882.1 | + | 13659 | 0.67 | 0.429934 |
Target: 5'- -gGCCGCuGGUgGGGCGAUcucacgcGCUaCGGCg -3' miRNA: 3'- ugCGGCG-UCAgCCCGUUAa------CGA-GCUG- -5' |
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27571 | 3' | -56.6 | NC_005882.1 | + | 22025 | 0.68 | 0.410479 |
Target: 5'- -gGCCGCGG-CGGuGCcaucgacgUGCUCGAUc -3' miRNA: 3'- ugCGGCGUCaGCC-CGuua-----ACGAGCUG- -5' |
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27571 | 3' | -56.6 | NC_005882.1 | + | 1337 | 0.68 | 0.410479 |
Target: 5'- aACGCCuGgAG-CGGGCcGUgccGCUCGAUg -3' miRNA: 3'- -UGCGG-CgUCaGCCCGuUAa--CGAGCUG- -5' |
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27571 | 3' | -56.6 | NC_005882.1 | + | 5322 | 0.68 | 0.400958 |
Target: 5'- uCGUCGguGcCGGGCGucaUGgUCGGCg -3' miRNA: 3'- uGCGGCguCaGCCCGUua-ACgAGCUG- -5' |
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27571 | 3' | -56.6 | NC_005882.1 | + | 1552 | 0.68 | 0.380515 |
Target: 5'- gACGCCcaucGCA-UCGGGCGucgccgcgcaGCUCGACg -3' miRNA: 3'- -UGCGG----CGUcAGCCCGUuaa-------CGAGCUG- -5' |
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27571 | 3' | -56.6 | NC_005882.1 | + | 24983 | 0.68 | 0.373257 |
Target: 5'- gACGCCGCGGagCGGGaaauCAAgcgGCgcgUCGACg -3' miRNA: 3'- -UGCGGCGUCa-GCCC----GUUaa-CG---AGCUG- -5' |
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27571 | 3' | -56.6 | NC_005882.1 | + | 27036 | 0.68 | 0.364316 |
Target: 5'- cGCGCUGCucccuuUCGGGCAGggcaUGCUgaUGACg -3' miRNA: 3'- -UGCGGCGuc----AGCCCGUUa---ACGA--GCUG- -5' |
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27571 | 3' | -56.6 | NC_005882.1 | + | 21087 | 0.68 | 0.364316 |
Target: 5'- gGCGUgugCGCAGcCGGcGCGAaggcGCUCGGCg -3' miRNA: 3'- -UGCG---GCGUCaGCC-CGUUaa--CGAGCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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