Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 27595 | 3' | -55.2 | NC_005882.1 | + | 29180 | 0.67 | 0.512462 |
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Target: 5'- --cCGGCGCGccguUGCUCGC-GU-GCGGCc -3' miRNA: 3'- uauGCUGUGU----ACGAGCGaCAcCGCCG- -5' |
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| 27595 | 3' | -55.2 | NC_005882.1 | + | 17807 | 0.67 | 0.512462 |
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Target: 5'- -aACGGCugGggcugccggUGC-CGC-GUGGUGGCg -3' miRNA: 3'- uaUGCUGugU---------ACGaGCGaCACCGCCG- -5' |
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| 27595 | 3' | -55.2 | NC_005882.1 | + | 12048 | 0.67 | 0.523423 |
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Target: 5'- -gACGGCACGUGCguugCGCgucagcucgGcGCGGUa -3' miRNA: 3'- uaUGCUGUGUACGa---GCGaca------C-CGCCG- -5' |
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| 27595 | 3' | -55.2 | NC_005882.1 | + | 5384 | 0.67 | 0.534472 |
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Target: 5'- -gACGGCGCcuugAUGagUUGCUG-GGCGGUa -3' miRNA: 3'- uaUGCUGUG----UACg-AGCGACaCCGCCG- -5' |
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| 27595 | 3' | -55.2 | NC_005882.1 | + | 4918 | 0.67 | 0.534472 |
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Target: 5'- -cGCGcCGCu--CUCGCUGaucgaGGCGGCa -3' miRNA: 3'- uaUGCuGUGuacGAGCGACa----CCGCCG- -5' |
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| 27595 | 3' | -55.2 | NC_005882.1 | + | 25763 | 0.66 | 0.556808 |
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Target: 5'- -cGCGAUgucgcgcagGCcgGCuUCGaagGUGGCGGCc -3' miRNA: 3'- uaUGCUG---------UGuaCG-AGCga-CACCGCCG- -5' |
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| 27595 | 3' | -55.2 | NC_005882.1 | + | 7696 | 0.66 | 0.568078 |
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Target: 5'- --uCGAgGC--GCUCGCg--GGCGGCg -3' miRNA: 3'- uauGCUgUGuaCGAGCGacaCCGCCG- -5' |
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| 27595 | 3' | -55.2 | NC_005882.1 | + | 27895 | 0.66 | 0.590777 |
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Target: 5'- uUGCGaACACGUcaGCUC-CUGUacGcGCGGCg -3' miRNA: 3'- uAUGC-UGUGUA--CGAGcGACA--C-CGCCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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