Results 21 - 23 of 23 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
27612 | 3' | -60.8 | NC_005882.1 | + | 23084 | 0.66 | 0.336487 |
Target: 5'- uCCGGCUgcGUUCAcGGcGCcgaugauguccgcgGCCGUGACCUu -3' miRNA: 3'- -GGCCGA--CAGGU-CCuCG--------------UGGCGCUGGA- -5' |
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27612 | 3' | -60.8 | NC_005882.1 | + | 3974 | 0.66 | 0.338907 |
Target: 5'- uCCgGGCUGcucuugaccucUCCAgcGGGGcCACgGCGGCCa -3' miRNA: 3'- -GG-CCGAC-----------AGGU--CCUC-GUGgCGCUGGa -5' |
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27612 | 3' | -60.8 | NC_005882.1 | + | 9732 | 0.66 | 0.354529 |
Target: 5'- gCCGGCUcaaUCGGGAGCGuugcuggUCGCGACa- -3' miRNA: 3'- -GGCCGAca-GGUCCUCGU-------GGCGCUGga -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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