miRNA display CGI


Results 21 - 27 of 27 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
27701 3' -57.3 NC_005882.1 + 17276 0.66 0.470293
Target:  5'- -aUCGCGCgggcaaGCCGUUCCU-GCAGcgcguCCg -3'
miRNA:   3'- uaGGCGUGa-----CGGCAAGGAgCGUCu----GG- -5'
27701 3' -57.3 NC_005882.1 + 12480 0.66 0.470293
Target:  5'- aAUCaCGCGCggguaGCCGcggUUCUCGCGGAa- -3'
miRNA:   3'- -UAG-GCGUGa----CGGCa--AGGAGCGUCUgg -5'
27701 3' -57.3 NC_005882.1 + 4693 0.66 0.470293
Target:  5'- -gCUGCAgcggUUGCUGUUgaccCgCUCGCGGGCCg -3'
miRNA:   3'- uaGGCGU----GACGGCAA----G-GAGCGUCUGG- -5'
27701 3' -57.3 NC_005882.1 + 14246 0.66 0.480631
Target:  5'- uUCCGCGCgUGCCugcaCCUC-CAGGCg -3'
miRNA:   3'- uAGGCGUG-ACGGcaa-GGAGcGUCUGg -5'
27701 3' -57.3 NC_005882.1 + 2039 0.66 0.491078
Target:  5'- -cUCGCAUUGCCuccagCCagCGCGGAUCg -3'
miRNA:   3'- uaGGCGUGACGGcaa--GGa-GCGUCUGG- -5'
27701 3' -57.3 NC_005882.1 + 5179 0.66 0.491078
Target:  5'- cGUCuCGCGC--CCG-UCCUCGCcagucaGGACCa -3'
miRNA:   3'- -UAG-GCGUGacGGCaAGGAGCG------UCUGG- -5'
27701 3' -57.3 NC_005882.1 + 29760 0.66 0.491078
Target:  5'- uUCCGCACggGCCGgcUCCaaggcaacgcUGCAGACg -3'
miRNA:   3'- uAGGCGUGa-CGGCa-AGGa---------GCGUCUGg -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.