Results 1 - 6 of 6 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
27724 | 5' | -46.8 | NC_005884.1 | + | 36018 | 1.08 | 0.005206 |
Target: 5'- gAUAUAGAAGGAACUGAUCCUAAGCCAa -3' miRNA: 3'- -UAUAUCUUCCUUGACUAGGAUUCGGU- -5' |
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27724 | 5' | -46.8 | NC_005884.1 | + | 35985 | 0.68 | 0.917961 |
Target: 5'- -cGUuGAAGc--CUGAUCCUGAGCCu -3' miRNA: 3'- uaUAuCUUCcuuGACUAGGAUUCGGu -5' |
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27724 | 5' | -46.8 | NC_005884.1 | + | 20997 | 0.68 | 0.93083 |
Target: 5'- -gGUGGAAGGAuGCUGAggagauUCCUcAGGCUu -3' miRNA: 3'- uaUAUCUUCCU-UGACU------AGGA-UUCGGu -5' |
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27724 | 5' | -46.8 | NC_005884.1 | + | 43112 | 0.67 | 0.947619 |
Target: 5'- --cUGGAGGGAcuAUUGAUCC--GGCUAa -3' miRNA: 3'- uauAUCUUCCU--UGACUAGGauUCGGU- -5' |
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27724 | 5' | -46.8 | NC_005884.1 | + | 34756 | 0.67 | 0.957167 |
Target: 5'- cAUAUAGAguuGGGcGGCUGcUCCaUAGGCCu -3' miRNA: 3'- -UAUAUCU---UCC-UUGACuAGG-AUUCGGu -5' |
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27724 | 5' | -46.8 | NC_005884.1 | + | 10382 | 0.66 | 0.965452 |
Target: 5'- -gGUGGAAGaacagccucGAAUaGAUCCUGAGgCCAa -3' miRNA: 3'- uaUAUCUUC---------CUUGaCUAGGAUUC-GGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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