miRNA display CGI


Results 1 - 6 of 6 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
27724 5' -46.8 NC_005884.1 + 10382 0.66 0.965452
Target:  5'- -gGUGGAAGaacagccucGAAUaGAUCCUGAGgCCAa -3'
miRNA:   3'- uaUAUCUUC---------CUUGaCUAGGAUUC-GGU- -5'
27724 5' -46.8 NC_005884.1 + 20997 0.68 0.93083
Target:  5'- -gGUGGAAGGAuGCUGAggagauUCCUcAGGCUu -3'
miRNA:   3'- uaUAUCUUCCU-UGACU------AGGA-UUCGGu -5'
27724 5' -46.8 NC_005884.1 + 34756 0.67 0.957167
Target:  5'- cAUAUAGAguuGGGcGGCUGcUCCaUAGGCCu -3'
miRNA:   3'- -UAUAUCU---UCC-UUGACuAGG-AUUCGGu -5'
27724 5' -46.8 NC_005884.1 + 35985 0.68 0.917961
Target:  5'- -cGUuGAAGc--CUGAUCCUGAGCCu -3'
miRNA:   3'- uaUAuCUUCcuuGACUAGGAUUCGGu -5'
27724 5' -46.8 NC_005884.1 + 36018 1.08 0.005206
Target:  5'- gAUAUAGAAGGAACUGAUCCUAAGCCAa -3'
miRNA:   3'- -UAUAUCUUCCUUGACUAGGAUUCGGU- -5'
27724 5' -46.8 NC_005884.1 + 43112 0.67 0.947619
Target:  5'- --cUGGAGGGAcuAUUGAUCC--GGCUAa -3'
miRNA:   3'- uauAUCUUCCU--UGACUAGGauUCGGU- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.