Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 27726 | 3' | -46.6 | NC_005884.1 | + | 8199 | 0.66 | 0.980407 |
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Target: 5'- gAUCUCCCUGGuCCGGc-UCAGgcAAUg -3' miRNA: 3'- -UAGAGGGAUC-GGUCuuAGUUauUUGa -5' |
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| 27726 | 3' | -46.6 | NC_005884.1 | + | 42386 | 0.66 | 0.980151 |
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Target: 5'- aAUCUCCCUaucuGGCCAGAcUgGAgauugaaaaggauUGGACUc -3' miRNA: 3'- -UAGAGGGA----UCGGUCUuAgUU-------------AUUUGA- -5' |
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| 27726 | 3' | -46.6 | NC_005884.1 | + | 5747 | 0.66 | 0.977735 |
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Target: 5'- uUCUCCgUAGCCuuGAGUCuGUAAc-- -3' miRNA: 3'- uAGAGGgAUCGGu-CUUAGuUAUUuga -5' |
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| 27726 | 3' | -46.6 | NC_005884.1 | + | 18424 | 0.66 | 0.971599 |
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Target: 5'- -cUUCCCaggguuaucgUAGCCAGAuUCAAUAAGg- -3' miRNA: 3'- uaGAGGG----------AUCGGUCUuAGUUAUUUga -5' |
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| 27726 | 3' | -46.6 | NC_005884.1 | + | 30102 | 0.66 | 0.971599 |
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Target: 5'- ---aCCgCUGGCCAGggUCAGgguuACa -3' miRNA: 3'- uagaGG-GAUCGGUCuuAGUUauu-UGa -5' |
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| 27726 | 3' | -46.6 | NC_005884.1 | + | 22302 | 0.67 | 0.964332 |
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Target: 5'- -cCUCCUUGGUCAGAAUCccguuuaucguAGUGAAgUa -3' miRNA: 3'- uaGAGGGAUCGGUCUUAG-----------UUAUUUgA- -5' |
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| 27726 | 3' | -46.6 | NC_005884.1 | + | 1304 | 0.68 | 0.935028 |
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Target: 5'- -gUUCCCUGGCCAGGAcuagccuaaUCGAagAGAUUa -3' miRNA: 3'- uaGAGGGAUCGGUCUU---------AGUUa-UUUGA- -5' |
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| 27726 | 3' | -46.6 | NC_005884.1 | + | 20188 | 0.7 | 0.868707 |
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Target: 5'- -gUUUCCUGGCCAcacuGAAUuCAGUAGACUc -3' miRNA: 3'- uaGAGGGAUCGGU----CUUA-GUUAUUUGA- -5' |
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| 27726 | 3' | -46.6 | NC_005884.1 | + | 42427 | 1.07 | 0.006222 |
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Target: 5'- aAUCUCCCUAGCCAGAAUCAAUAAACUg -3' miRNA: 3'- -UAGAGGGAUCGGUCUUAGUUAUUUGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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