Results 1 - 6 of 6 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
27737 | 5' | -52.1 | NC_005884.1 | + | 17549 | 0.66 | 0.800324 |
Target: 5'- cAGGGUCUcGaCUCAuuACUUCGCUGACCu -3' miRNA: 3'- -UUUCAGA-C-GAGUc-UGAAGCGGUUGGc -5' |
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27737 | 5' | -52.1 | NC_005884.1 | + | 36649 | 1.06 | 0.002071 |
Target: 5'- uAAAGUCUGCUCAGACUUCGCCAACCGu -3' miRNA: 3'- -UUUCAGACGAGUCUGAAGCGGUUGGC- -5' |
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27737 | 5' | -52.1 | NC_005884.1 | + | 5428 | 0.71 | 0.464944 |
Target: 5'- cGGGUUUGagaCUgAGACUUCGUCAACCa -3' miRNA: 3'- uUUCAGAC---GAgUCUGAAGCGGUUGGc -5' |
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27737 | 5' | -52.1 | NC_005884.1 | + | 23943 | 0.68 | 0.645102 |
Target: 5'- gAAGGUaaggaGCUCAG-CUUCGCCAgACCu -3' miRNA: 3'- -UUUCAga---CGAGUCuGAAGCGGU-UGGc -5' |
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27737 | 5' | -52.1 | NC_005884.1 | + | 21764 | 0.66 | 0.757225 |
Target: 5'- -cAGUCUGUUauuaaCAGGCUUUcugguagggauaaGCCAGCCa -3' miRNA: 3'- uuUCAGACGA-----GUCUGAAG-------------CGGUUGGc -5' |
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27737 | 5' | -52.1 | NC_005884.1 | + | 18863 | 0.66 | 0.810371 |
Target: 5'- ----aCUGCUCAGACUggGaCAGCUGa -3' miRNA: 3'- uuucaGACGAGUCUGAagCgGUUGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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