Results 1 - 20 of 32 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
27922 | 5' | -45.4 | NC_005886.2 | + | 30651 | 0.66 | 0.996344 |
Target: 5'- uAGgCGUUGCuuucauGAUGAAuGUAUGCCu -3' miRNA: 3'- -UCgGCGACGuu----UUGCUUuUAUAUGGc -5' |
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27922 | 5' | -45.4 | NC_005886.2 | + | 29018 | 0.66 | 0.994786 |
Target: 5'- cGCCgacauGCUGCGAcGCGAuguucAGAUAUAgCGu -3' miRNA: 3'- uCGG-----CGACGUUuUGCU-----UUUAUAUgGC- -5' |
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27922 | 5' | -45.4 | NC_005886.2 | + | 22913 | 0.66 | 0.994786 |
Target: 5'- cGGUCGCuuucgUGCAGAacucggGCGAuAGcGUGCCGg -3' miRNA: 3'- -UCGGCG-----ACGUUU------UGCUuUUaUAUGGC- -5' |
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27922 | 5' | -45.4 | NC_005886.2 | + | 11363 | 0.66 | 0.994786 |
Target: 5'- -uCCGCcaGCGAAGCGAGGAa--ACCGg -3' miRNA: 3'- ucGGCGa-CGUUUUGCUUUUauaUGGC- -5' |
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27922 | 5' | -45.4 | NC_005886.2 | + | 34726 | 0.66 | 0.994786 |
Target: 5'- cAGCCGgUGCAGcACGuucAAUAUcggACCa -3' miRNA: 3'- -UCGGCgACGUUuUGCuu-UUAUA---UGGc -5' |
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27922 | 5' | -45.4 | NC_005886.2 | + | 15896 | 0.66 | 0.994512 |
Target: 5'- uGCCGUUGCGGAacacacgucggagcGCGAuccguccgcgccGAucGUACCGg -3' miRNA: 3'- uCGGCGACGUUU--------------UGCU------------UUuaUAUGGC- -5' |
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27922 | 5' | -45.4 | NC_005886.2 | + | 42941 | 0.66 | 0.993827 |
Target: 5'- cAGuuGCgGCAuuACGAAAugaaACCGg -3' miRNA: 3'- -UCggCGaCGUuuUGCUUUuauaUGGC- -5' |
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27922 | 5' | -45.4 | NC_005886.2 | + | 44604 | 0.66 | 0.993827 |
Target: 5'- aAGCCGUgGCcGAGCGAuggcaaGCCGa -3' miRNA: 3'- -UCGGCGaCGuUUUGCUuuuauaUGGC- -5' |
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27922 | 5' | -45.4 | NC_005886.2 | + | 34543 | 0.67 | 0.991484 |
Target: 5'- cGCUGCgcgUGCGGggUGGAuuugAUGCCGu -3' miRNA: 3'- uCGGCG---ACGUUuuGCUUuua-UAUGGC- -5' |
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27922 | 5' | -45.4 | NC_005886.2 | + | 45074 | 0.67 | 0.990074 |
Target: 5'- uAGCCG-UGCGGAACGAAAcgcgGCUu -3' miRNA: 3'- -UCGGCgACGUUUUGCUUUuauaUGGc -5' |
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27922 | 5' | -45.4 | NC_005886.2 | + | 10221 | 0.67 | 0.988488 |
Target: 5'- cAGCCGCUGCAcuaaaGAAGGcagGUACa- -3' miRNA: 3'- -UCGGCGACGUuuug-CUUUUa--UAUGgc -5' |
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27922 | 5' | -45.4 | NC_005886.2 | + | 35585 | 0.67 | 0.986712 |
Target: 5'- uGCUGCacGUAAAGCGgcGggAUACCGg -3' miRNA: 3'- uCGGCGa-CGUUUUGCuuUuaUAUGGC- -5' |
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27922 | 5' | -45.4 | NC_005886.2 | + | 32530 | 0.68 | 0.983442 |
Target: 5'- cGGCCGCUGCAAAcccugcauGCGcugcacgcACCGc -3' miRNA: 3'- -UCGGCGACGUUU--------UGCuuuuaua-UGGC- -5' |
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27922 | 5' | -45.4 | NC_005886.2 | + | 29298 | 0.68 | 0.977442 |
Target: 5'- cGGCCGCUGC-AAACGc------GCCGu -3' miRNA: 3'- -UCGGCGACGuUUUGCuuuuauaUGGC- -5' |
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27922 | 5' | -45.4 | NC_005886.2 | + | 46203 | 0.68 | 0.974518 |
Target: 5'- cGGCaagaaaGUUGCAGacaguGACGGAucUGUGCCGa -3' miRNA: 3'- -UCGg-----CGACGUU-----UUGCUUuuAUAUGGC- -5' |
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27922 | 5' | -45.4 | NC_005886.2 | + | 13298 | 0.68 | 0.974518 |
Target: 5'- uGCCGCcG-AAAACGAAAugcccgAUACCGu -3' miRNA: 3'- uCGGCGaCgUUUUGCUUUua----UAUGGC- -5' |
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27922 | 5' | -45.4 | NC_005886.2 | + | 47262 | 0.69 | 0.971328 |
Target: 5'- uGCCGCUG----GCGAAcagguUAUGCCGa -3' miRNA: 3'- uCGGCGACguuuUGCUUuu---AUAUGGC- -5' |
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27922 | 5' | -45.4 | NC_005886.2 | + | 12587 | 0.69 | 0.971328 |
Target: 5'- cGCCGUcuUGaUAGAACGAAAGauucGUACCGu -3' miRNA: 3'- uCGGCG--AC-GUUUUGCUUUUa---UAUGGC- -5' |
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27922 | 5' | -45.4 | NC_005886.2 | + | 38712 | 0.69 | 0.964107 |
Target: 5'- cAGCCGCUGUuucAGAucguCGAccAUGUugCGg -3' miRNA: 3'- -UCGGCGACG---UUUu---GCUuuUAUAugGC- -5' |
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27922 | 5' | -45.4 | NC_005886.2 | + | 10441 | 0.69 | 0.962923 |
Target: 5'- uGCCGCUGCuuccgcuuguugccGCGuGAcgAUGCCGa -3' miRNA: 3'- uCGGCGACGuuu-----------UGCuUUuaUAUGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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