Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 27926 | 5' | -49.7 | NC_005886.2 | + | 32443 | 0.72 | 0.651165 |
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Target: 5'- -cCCACugUCGCGCGuGUUGuc-GGCCg -3' miRNA: 3'- acGGUGugAGCGUGC-UAACuuuCUGG- -5' |
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| 27926 | 5' | -49.7 | NC_005886.2 | + | 32986 | 0.72 | 0.628046 |
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Target: 5'- cGUCGCGCgUUGCGCG---GGAAGACCu -3' miRNA: 3'- aCGGUGUG-AGCGUGCuaaCUUUCUGG- -5' |
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| 27926 | 5' | -49.7 | NC_005886.2 | + | 27838 | 0.74 | 0.547809 |
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Target: 5'- uUGCCACGCgccgGUACGGUUGAuccGGGCa -3' miRNA: 3'- -ACGGUGUGag--CGUGCUAACUu--UCUGg -5' |
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| 27926 | 5' | -49.7 | NC_005886.2 | + | 23021 | 0.74 | 0.525404 |
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Target: 5'- uUGCUGCGCUCGguUACGAUccgaUGGAAGGCg -3' miRNA: 3'- -ACGGUGUGAGC--GUGCUA----ACUUUCUGg -5' |
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| 27926 | 5' | -49.7 | NC_005886.2 | + | 13562 | 0.76 | 0.419732 |
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Target: 5'- cGCCGCACUCGuUGCGGUgaucagccGAucGACCg -3' miRNA: 3'- aCGGUGUGAGC-GUGCUAa-------CUuuCUGG- -5' |
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| 27926 | 5' | -49.7 | NC_005886.2 | + | 14963 | 1.13 | 0.001839 |
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Target: 5'- aUGCCACACUCGCACGAUUGAAAGACCc -3' miRNA: 3'- -ACGGUGUGAGCGUGCUAACUUUCUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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