Results 1 - 20 of 25 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
27927 | 5' | -53 | NC_005886.2 | + | 13574 | 0.66 | 0.844394 |
Target: 5'- uGCgGUGAUCAgcCGaUCGACCGACa- -3' miRNA: 3'- gCG-CGCUAGUaaGUaGGCUGGCUGcu -5' |
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27927 | 5' | -53 | NC_005886.2 | + | 26690 | 0.66 | 0.844394 |
Target: 5'- cCGCGCGAgccgcUUGUUgcCGUCCGAUucgagcgugaCGGCGAa -3' miRNA: 3'- -GCGCGCU-----AGUAA--GUAGGCUG----------GCUGCU- -5' |
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27927 | 5' | -53 | NC_005886.2 | + | 45927 | 0.66 | 0.844394 |
Target: 5'- gCGCGCGAUCug-CG-CCGAUgCGGCu- -3' miRNA: 3'- -GCGCGCUAGuaaGUaGGCUG-GCUGcu -5' |
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27927 | 5' | -53 | NC_005886.2 | + | 35963 | 0.66 | 0.822711 |
Target: 5'- aGCGcCGAUCGUUCGUCgUGAUCGuuaaaccgugcuuCGAu -3' miRNA: 3'- gCGC-GCUAGUAAGUAG-GCUGGCu------------GCU- -5' |
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27927 | 5' | -53 | NC_005886.2 | + | 15072 | 0.66 | 0.817101 |
Target: 5'- cCGCGCGAUgGUgcgccgaauUCAUguaUCGACgCGACGu -3' miRNA: 3'- -GCGCGCUAgUA---------AGUA---GGCUG-GCUGCu -5' |
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27927 | 5' | -53 | NC_005886.2 | + | 21814 | 0.66 | 0.807591 |
Target: 5'- gCGUaGCGAUCAgggcgcaCAUCUGAugcCCGAUGAa -3' miRNA: 3'- -GCG-CGCUAGUaa-----GUAGGCU---GGCUGCU- -5' |
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27927 | 5' | -53 | NC_005886.2 | + | 28067 | 0.66 | 0.807591 |
Target: 5'- aGCGCGAUCGUggaaagGUCCG-CaCGGCa- -3' miRNA: 3'- gCGCGCUAGUAag----UAGGCuG-GCUGcu -5' |
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27927 | 5' | -53 | NC_005886.2 | + | 21987 | 0.66 | 0.803734 |
Target: 5'- uCGCGUGAUCAcgaaucgccaucaUCGUCUGAUgGACa- -3' miRNA: 3'- -GCGCGCUAGUa------------AGUAGGCUGgCUGcu -5' |
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27927 | 5' | -53 | NC_005886.2 | + | 24522 | 0.66 | 0.797892 |
Target: 5'- uGCGCGG-CG-UCA-CUGGCCGGCGc -3' miRNA: 3'- gCGCGCUaGUaAGUaGGCUGGCUGCu -5' |
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27927 | 5' | -53 | NC_005886.2 | + | 34004 | 0.66 | 0.797892 |
Target: 5'- cCGCGcCGAcCGaaugguUUCcgCCGACCGuCGAa -3' miRNA: 3'- -GCGC-GCUaGU------AAGuaGGCUGGCuGCU- -5' |
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27927 | 5' | -53 | NC_005886.2 | + | 38864 | 0.67 | 0.777975 |
Target: 5'- --aGCGAUCAUggcCGUCCGuCgCGACGu -3' miRNA: 3'- gcgCGCUAGUAa--GUAGGCuG-GCUGCu -5' |
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27927 | 5' | -53 | NC_005886.2 | + | 9741 | 0.67 | 0.776962 |
Target: 5'- -cCGCGAUCAauUUCAcuaccacUCaacuuGACCGACGAg -3' miRNA: 3'- gcGCGCUAGU--AAGU-------AGg----CUGGCUGCU- -5' |
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27927 | 5' | -53 | NC_005886.2 | + | 34712 | 0.68 | 0.736375 |
Target: 5'- cCGUGCGAUCGUUCcgCgcgggugGACgUGACGAu -3' miRNA: 3'- -GCGCGCUAGUAAGuaGg------CUG-GCUGCU- -5' |
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27927 | 5' | -53 | NC_005886.2 | + | 22577 | 0.68 | 0.714883 |
Target: 5'- aGCGCGAgcaCAcgccCGUCCGACUGccguGCGAa -3' miRNA: 3'- gCGCGCUa--GUaa--GUAGGCUGGC----UGCU- -5' |
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27927 | 5' | -53 | NC_005886.2 | + | 23480 | 0.69 | 0.670989 |
Target: 5'- uCGCGCGGUCGUUgAgaagCUGGaauacgauCCGAUGAa -3' miRNA: 3'- -GCGCGCUAGUAAgUa---GGCU--------GGCUGCU- -5' |
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27927 | 5' | -53 | NC_005886.2 | + | 33042 | 0.69 | 0.648771 |
Target: 5'- aCGCGCGA-CGUUC-UCUGAUcuguaCGACGGu -3' miRNA: 3'- -GCGCGCUaGUAAGuAGGCUG-----GCUGCU- -5' |
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27927 | 5' | -53 | NC_005886.2 | + | 12636 | 0.7 | 0.615351 |
Target: 5'- gGCGUGAUgAUgggCgGUCCGACCGAUc- -3' miRNA: 3'- gCGCGCUAgUAa--G-UAGGCUGGCUGcu -5' |
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27927 | 5' | -53 | NC_005886.2 | + | 15873 | 0.71 | 0.560073 |
Target: 5'- aGCGCGAUCcgUCcgcgCCGAUCGuacCGGa -3' miRNA: 3'- gCGCGCUAGuaAGua--GGCUGGCu--GCU- -5' |
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27927 | 5' | -53 | NC_005886.2 | + | 14953 | 0.71 | 0.538317 |
Target: 5'- aGCGCuugcucaaCAUUC-UCUGACCGACGAc -3' miRNA: 3'- gCGCGcua-----GUAAGuAGGCUGGCUGCU- -5' |
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27927 | 5' | -53 | NC_005886.2 | + | 38769 | 0.72 | 0.495749 |
Target: 5'- cCGCaCGAUCcgcUCAUCC-ACCGGCGAg -3' miRNA: 3'- -GCGcGCUAGua-AGUAGGcUGGCUGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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