Results 21 - 25 of 25 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
27927 | 5' | -53 | NC_005886.2 | + | 15072 | 0.66 | 0.817101 |
Target: 5'- cCGCGCGAUgGUgcgccgaauUCAUguaUCGACgCGACGu -3' miRNA: 3'- -GCGCGCUAgUA---------AGUA---GGCUG-GCUGCu -5' |
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27927 | 5' | -53 | NC_005886.2 | + | 35963 | 0.66 | 0.822711 |
Target: 5'- aGCGcCGAUCGUUCGUCgUGAUCGuuaaaccgugcuuCGAu -3' miRNA: 3'- gCGC-GCUAGUAAGUAG-GCUGGCu------------GCU- -5' |
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27927 | 5' | -53 | NC_005886.2 | + | 45927 | 0.66 | 0.844394 |
Target: 5'- gCGCGCGAUCug-CG-CCGAUgCGGCu- -3' miRNA: 3'- -GCGCGCUAGuaaGUaGGCUG-GCUGcu -5' |
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27927 | 5' | -53 | NC_005886.2 | + | 13574 | 0.66 | 0.844394 |
Target: 5'- uGCgGUGAUCAgcCGaUCGACCGACa- -3' miRNA: 3'- gCG-CGCUAGUaaGUaGGCUGGCUGcu -5' |
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27927 | 5' | -53 | NC_005886.2 | + | 26690 | 0.66 | 0.844394 |
Target: 5'- cCGCGCGAgccgcUUGUUgcCGUCCGAUucgagcgugaCGGCGAa -3' miRNA: 3'- -GCGCGCU-----AGUAA--GUAGGCUG----------GCUGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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