Results 1 - 20 of 20 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
27935 | 5' | -52.8 | NC_005886.2 | + | 1071 | 0.66 | 0.803991 |
Target: 5'- gGAUGGAugcacAAGCCG-UCGCGgGUGCa-- -3' miRNA: 3'- -CUGCUU-----UUCGGCaAGUGCgCACGcgu -5' |
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27935 | 5' | -52.8 | NC_005886.2 | + | 4158 | 0.66 | 0.803991 |
Target: 5'- -uCGuAAAGCCGgcacguggGCGCGUGCGUc -3' miRNA: 3'- cuGCuUUUCGGCaag-----UGCGCACGCGu -5' |
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27935 | 5' | -52.8 | NC_005886.2 | + | 35599 | 0.66 | 0.803991 |
Target: 5'- cGGCGGGAuaccGGCCcaugcguccaaGUauUCGcCGCGUGCGCu -3' miRNA: 3'- -CUGCUUU----UCGG-----------CA--AGU-GCGCACGCGu -5' |
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27935 | 5' | -52.8 | NC_005886.2 | + | 32196 | 0.66 | 0.794031 |
Target: 5'- --aGAaacuGAAGCUGUUCGgGCGU-CGCAg -3' miRNA: 3'- cugCU----UUUCGGCAAGUgCGCAcGCGU- -5' |
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27935 | 5' | -52.8 | NC_005886.2 | + | 10137 | 0.66 | 0.794031 |
Target: 5'- uGCGAAAaaauGGCCGUggUUugGUgauacgGUGCGCGc -3' miRNA: 3'- cUGCUUU----UCGGCA--AGugCG------CACGCGU- -5' |
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27935 | 5' | -52.8 | NC_005886.2 | + | 38190 | 0.66 | 0.783892 |
Target: 5'- uGAUGAAcgucaguugcGCCGU---CGCGUGCGCAu -3' miRNA: 3'- -CUGCUUuu--------CGGCAaguGCGCACGCGU- -5' |
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27935 | 5' | -52.8 | NC_005886.2 | + | 46888 | 0.66 | 0.773585 |
Target: 5'- cGACGAGugcGCCGgcaagCAUGCGUucgacuGCGCu -3' miRNA: 3'- -CUGCUUuu-CGGCaa---GUGCGCA------CGCGu -5' |
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27935 | 5' | -52.8 | NC_005886.2 | + | 31109 | 0.66 | 0.771504 |
Target: 5'- -uCGGAAgcuugccGGCCGcaaagggUUCAaGCGUGCGCAa -3' miRNA: 3'- cuGCUUU-------UCGGC-------AAGUgCGCACGCGU- -5' |
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27935 | 5' | -52.8 | NC_005886.2 | + | 21629 | 0.67 | 0.735279 |
Target: 5'- uAUGAAAAGCCGUUUaauaucgacggggugAUGCccgGUGCGUAu -3' miRNA: 3'- cUGCUUUUCGGCAAG---------------UGCG---CACGCGU- -5' |
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27935 | 5' | -52.8 | NC_005886.2 | + | 29276 | 0.67 | 0.70891 |
Target: 5'- cGAUGA--AGCCGcu-GCGCGUGCGgGu -3' miRNA: 3'- -CUGCUuuUCGGCaagUGCGCACGCgU- -5' |
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27935 | 5' | -52.8 | NC_005886.2 | + | 37033 | 0.68 | 0.697776 |
Target: 5'- cGACGAGuccuGCCG-UCACGCcUGcCGCu -3' miRNA: 3'- -CUGCUUuu--CGGCaAGUGCGcAC-GCGu -5' |
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27935 | 5' | -52.8 | NC_005886.2 | + | 24844 | 0.68 | 0.652686 |
Target: 5'- cGCGc-GAGCCGgUCGCGCGggGCGUu -3' miRNA: 3'- cUGCuuUUCGGCaAGUGCGCa-CGCGu -5' |
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27935 | 5' | -52.8 | NC_005886.2 | + | 32721 | 0.69 | 0.628836 |
Target: 5'- cGCGAAcgaGAGCCGaaCACgucgcguccuucgGCGUGCGCc -3' miRNA: 3'- cUGCUU---UUCGGCaaGUG-------------CGCACGCGu -5' |
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27935 | 5' | -52.8 | NC_005886.2 | + | 9047 | 0.69 | 0.618614 |
Target: 5'- gGGCGAAcAGuuGUUCACGCaaGCGgAa -3' miRNA: 3'- -CUGCUUuUCggCAAGUGCGcaCGCgU- -5' |
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27935 | 5' | -52.8 | NC_005886.2 | + | 25170 | 0.69 | 0.595944 |
Target: 5'- -uCGAu--GCCGUUCACGCGaUGCa-- -3' miRNA: 3'- cuGCUuuuCGGCAAGUGCGC-ACGcgu -5' |
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27935 | 5' | -52.8 | NC_005886.2 | + | 41364 | 0.7 | 0.562206 |
Target: 5'- uGCGAAcuGCCGuUUCGgGCGUcGCGCc -3' miRNA: 3'- cUGCUUuuCGGC-AAGUgCGCA-CGCGu -5' |
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27935 | 5' | -52.8 | NC_005886.2 | + | 41217 | 0.7 | 0.562206 |
Target: 5'- cGACGAAGAaCCGUU-GCGCGaUGCGUc -3' miRNA: 3'- -CUGCUUUUcGGCAAgUGCGC-ACGCGu -5' |
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27935 | 5' | -52.8 | NC_005886.2 | + | 35474 | 0.71 | 0.496556 |
Target: 5'- -cUGAGAAGCCGUcgcaccUCACGaauagcuucaGUGCGCGu -3' miRNA: 3'- cuGCUUUUCGGCA------AGUGCg---------CACGCGU- -5' |
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27935 | 5' | -52.8 | NC_005886.2 | + | 23116 | 0.76 | 0.278934 |
Target: 5'- cGACGgcGAGCCGUUCACGU--GCGUu -3' miRNA: 3'- -CUGCuuUUCGGCAAGUGCGcaCGCGu -5' |
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27935 | 5' | -52.8 | NC_005886.2 | + | 22101 | 1.09 | 0.001339 |
Target: 5'- cGACGAAAAGCCGUUCACGCGUGCGCAa -3' miRNA: 3'- -CUGCUUUUCGGCAAGUGCGCACGCGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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